镉对大鼠附睾的损伤及锌和维生素 E 的保护作用

ULTRASTRUCTURAL AND AlPase CYTOCHEMICAL STUDIES OF INJURIOUS ACTION OF CADMIUM AND PROTECTION OF ZINC AND VITAMIN E ON RAT EPIDIDYMIDES

用透射电镜结合脂质过氧化物（ＬＰＯ）含量分析法，观察小剂量氯化镉（２ｍｇ／ｋｇ体重）腹腔内注射后１０ｍｉｎ至６０ｄ大鼠附睾头部的损伤改变及锌和维生素Ｅ（ＶＥ）的保护作用。结果表明：注射镉后３０ｍｉｎ，主细胞已出现超微结构改变；３～７ｄ时主细胞和管周组织及毛细血管退变加重；１５～３０ｄ时退变有所减轻；６０ｄ时主细胞有恢复趋势而管周组织增厚。镉注射后附睾管周组织内碱性磷酸酶（ＡｌＰａｓｅ）反应产物明显减少。锌和ＶＥ对镉所致的形态损伤和酶活性下降有保护作用，且锌的效果优于ＶＥ。本实验还证实镉可使附睾ＬＰＯ含量增高，ＶＥ能较好颉颃脂质过氧化作用。

The ultrastructural alterations of principal cells and alkaline phosphatase(AlPase) activity of the peritubular tissue as well as the levels of lipid peroxide in epididymal tissue were studied.The rats were divided into several groups at different interval from 10 minutes to 2 months after treatment with small dose of CdCl 2 (2mg/kg body weight).Meanwhile,the protective effects of zinc(Zn) and Vitamin E on cadmium(Cd) induced injury were observed.The results showed that the ultrastructural changes of principal cells were detected after 30 minutes.After 3 7 days,the degenerations of principal cells,peritubular tissue and capillaries became prominent.They appeared to be alleviated after 15 30 days,and tend to recovered after 60 days.AlPase reaction products of peritubular tissue were decreased prominently after Cd treatment.Zn and Vitamin E were able to prevent the injury of cell structure and the inhibition of AlPase activities from Cd.The action of Zn was greater than that of Vitamin E.In addition,this paper confirmed that Cd resulted in a high level of lipid peroxide in epididymides.Vitamin E could be obviously against lipid peroxidation from Cd.