期刊文献+

工频磁场对离体细胞胞浆钙离子浓度的影响

Effects of 50 Hz Ferromagnetic Field on the Concentrations of Intracellular Calcium-ion in vitro
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摘要 研究了50 Hz工频均匀强磁场(0.097 T)对人低分化胃腺癌细胞株—SNU细胞和人淋巴细胞胞浆钙离子浓度的影响.将体外培养的SNU细胞和人淋巴细胞,施加50 Hz工频均匀强磁场(0.097 T),处理不同时间后,应用共聚焦显微镜检测胞浆内钙离子浓度.实验表明,给予工频均匀强磁场处理后,SNU细胞和人淋巴细胞胞浆内钙离子浓度均升高. The study was designed to observe the effects of 50 Hz homogeneous ferromagnetic field (0.097 T) exposure on change of intracellular calcium-ion concentration of human low differentiation gastric adenocarcinoma cell line-SNU and lymphocytes in vitro. SNU cells and human peripheral blood lymphocytes in vitro were exposured to 50 Hz homogeneous ferromagnetic field at different time peroids. Cells were harvested after magnetic field exposure. The concentration of intracellular calcium-ion of cells in control and different experimental groups were measured with confocal microscope. The concentration of intracellular calcium-ion of SNU cells and human peripheral blood lymphocytes were all increased after magnetic field exposure.
出处 《河北大学学报(自然科学版)》 CAS 北大核心 2008年第1期34-38,共5页 Journal of Hebei University(Natural Science Edition)
基金 河北省自然科学基金资助项目(502046)
关键词 工频均匀磁场 SNU细胞 人淋巴细胞 钙离子浓度 共聚焦显微镜 homogeneous ferromagnetic field SNU cells human lymphocytes calcium-ion concentration confocal microscope
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参考文献12

  • 1章国来,曾星,梅玉屏,何盛琪,张新梅,黄羽.猪苓多糖对膀胱癌细胞钙离子浓度的影响[J].中国临床药理学与治疗学,2001,6(3):204-206. 被引量:6
  • 2杨巍,徐韬,霍小林,宋涛.极低频磁场对HepG2细胞内游离钙离子浓度的影响[J].中华劳动卫生职业病杂志,2003,21(5):332-334. 被引量:4
  • 3BARBIER E, DUFY B, VEYRET B. Stimulation of Ca^2+ influx in rat pituitary cells under exposure to a 50 Hz magnetic fields [ J ]. Bioelectromagnetics, 1996,17: 303 - 311.
  • 4PESSINA GP, ALDIUUCCI C, PALMI M, et al. Pulsed electromagnetic fields affect the intracellular calcium concentration in human astrocytoma cells[ J ]. Bioelectromagnetics, 2001,22 : 503 - 510.
  • 5MC CREARY CR, THOMAS A W, PRATO F S. Factors confounding cytosolic calcium measurements in Jurkat E 6.1 cells during exposure to ELF magnetic fields[J]. Bioelectromagnetics, 2002, 23:315- 328.
  • 6IKEHARA T, PARK KH, YAMAGUCHI H, et al. Effects of a time varying strong magnetic field on release of cytosolic free Ca^2+ from intracellular stores in cultured bovinc adrenal chromaffin cells[J]. Bioelectromagnetics, 2002,23:505- 515.
  • 7WEY H E, CONOVER D P, MATHIAS P, et al. 50 Hz magnetic field and calcium transients in Jurkat cells: results of a research and public information dissemination(RAPID) program study[J]. Environ Health Perspect, 2000,108:135- 140.
  • 8SHAHI DAIN R, MULLINS R D, SISKEN J E. Calcium spiking activity and baseline calcium levels in ROS 17/2.8 cells exposed to extremely low frequency electromagnetic fields[ J ]. Int J Radiat Biol, 2001,77 (2) : 241 - 248.
  • 9陈建庭,谭小云,杨春露,唐焕章,金大地.脉冲电磁场对成骨细胞-破骨细胞复合培养体系中成骨细胞膜电位及细胞内钙离子的影响[J].中国临床康复,2004,8(5):870-872. 被引量:9
  • 10TAKEMI T, MOUSTAKAS A, LIN H Y, et al. Effects of 10-T static magnetic field on human peripheral blood immune cells [ J 1. Radiat Res, 2003,159 (6) : 775 - 779.

二级参考文献24

  • 1李波,徐红薇,田景先.猪苓多糖对LAK细胞杀伤肿瘤活性的调节作用[J].中国肿瘤生物治疗杂志,1995,2(4):349-350. 被引量:2
  • 2Wertheimer N, Savitz DA, Leeper E. Childhood cancer in relation to indicators of magnetic fields from ground current sources. Bioelectromagnetics, 1995,16: 86-96.
  • 3Adey WR. Cell membranes : the electromagnetic environment and cancer promotion. Neurochem Res, 1988,13:671-677.
  • 4Tsong TY. Electrical modulation of membrane proteins:enforced conformational oscillations and biological energy and signal transductions. Annu Rev Biophys Chem, 1990,19:83-106.
  • 5Walleczek J. Electromagnetic field effects on cells of the immune system: the role of calcium signaling. FASEB J, 1992,6: 3177-3185.
  • 6Barbier E, Dufy B, Veyret B. Stimulation of Ca^2+ influx in rat pituitary cells under exposure to a 50 Hz magnetic fields. Bioelectromagnetica,1996,17:303-311.
  • 7Pessina GP, Aldinucci C, Palmi M, et al. Pulsed electromgnetic fields affect the intracellular calcium concentrations in human astrocytonma cells. Bioelectromagnetics, 2001,22 : 503-510.
  • 8McCreary CR, Thomas AW, Prato FS. Factors confounding cytosolic calcium measurements in Jurkat E6.1 cells during exposure to ELF magnetic fields. Bioelectromagnetica,2002,23:315-328.
  • 9Ikehara T, Park KH, Yamaguchi H, et al. Effects of a time varying strong magnetic field on release of cytosolie free Ca^2+ from intracellular stores in cultured bovine adrenal chromaffin cells. Bioelectromagnetics,2002,23 : 505-515.
  • 10Sontag W. Action of extremely low frequency electric fields on the cytosolic calcium concentration of differentiated HL-60 cells : nonactivated cells. Bioelectromagnetics, 1998,19 : 32-40.

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