同步荧光-双波长法同时测定血浆中儿茶酚胺类神经递质

Simultaneous Determination of Catecholamines Neurotransmitters in Blood Plasma by Synchronous Scanning-dual Wavelength Fluorimetry

建立一种同步荧光法与双波长法结合起来同时测定血浆中肾上腺素（E）、去甲肾上腺素（NE）和多巴胺（DA）3种儿茶酚胺类神经递质的方法．对E，NE和DA3种神经递质的衍生物分别进行同步荧光扫描，考察影响体系荧光强度的因素．△λ=70nm时获得的同步荧光光谱图中，DA在385．0nm处的荧光信号不受干扰，而且E和NE的相互干扰可通过双波长法消除．最佳实验条件：0．5mol／L乙酸-乙酸钠为缓冲液（pH=6．5），E，NE和DA的加热时间分别为1，3和35min．E，NE和DA线性范围分别为0～320，0～640μg／L和0～160μg／L，相关系数分别为0．9995，0．9998和0．9993；最低检测限分别为0．20，0．97μg／L和0．73μg／L；血浆样品经酸性正丁醇和正庚烷进行处理．该法用于血浆中儿茶酚胺类神经递质的测定，结果令人满意．

A synchronous scanning-dual wavelength fluorimetric method for simultaneous determination of epinephrine, norepinephrine and dopamine in blood plasma has been described. The ramifications of E,NE and DA have been scanned simultaneously to examine the factors which affect the intensity of systematic fluorescence. When △λ was 70 nm, the largest fluorescence signal of DA, which was 385 nm, was not disturbed in the simultaneous fluorescence spectrum gained and the mutual interference of E and NE could be eliminated by the dual-wavelength method. The largest fluorescence intensity could be got in 0.5 mol/L acetic acid - sodium acetate buffer at pH= 6.5 and the heating time of E, NE and DA were 1 rain, 3 min and 35 min respectivelv.The linear ranges were 0- 320 μg/L （ r = 0. 999 5） for epinephrine, 0-640 μg/L （ r = 0. 999 8） for norepinephrine and 0-160 μg/L （r = 0. 999 3） for dopamine. The detection limits of E, NE and DA were 0.20, 0.97 μg/L and 0.73 μg/L, respectively. The method has been applied to determine the contents of catecholamines neurotransmitters in blood plasma with satisfactory results.