芍药苷抑制NF-κB的活性促进人胃癌细胞凋亡

Paeoniflorin induces the apoptosis of human gastric carcinoma cells by inhibiting activation of NF-κB

目的:探讨中药白芍的有效成分——芍药苷对人胃癌SGC-7901细胞株NF-κB的活性以及细胞凋亡的影响。方法:将芍药苷以不同浓度及不同时间作用于胃癌SGC-7901细胞。应用WesternBlot方法检测各组胞核NF-κB表达情况,并应用胞核NF-κB活性检测(ELISA法)进一步确定胞核内NF-κB的表达情况,流式细胞仪检测联合应用芍药苷及5-氟尿嘧啶(5-FU)对胃癌细胞凋亡的影响。结果:芍药苷对胃癌SGC-7901细胞胞核NF-κB表达有明确的抑制作用,WesternBlot以及胞核NF-κB活性检测结果均显示这种抑制存在着时间及剂量依赖现象,流式细胞术检测结果表明芍药苷对5-FU诱导的肿瘤细胞凋亡有明显的促进作用。结论:芍药苷抑制了细胞内NF-κB的表达,并借助这种作用增强5-FU诱导的细胞凋亡效应。

Objective：To investigate Paeoniflorin＇s inhibition effect on NF-κB in human gastric carcinoma cells and its role in the induction of cell apoptosis. Methods： Paeoniflorin was diluted to different concentrations and treated on the gastric carcinoma ceils at different time. Western Blot was used to test the expression of NF-κB in nuclear and cytoplasm. The intranuclear expression of NF-κB was confirmed by ELISA assay. The influence on cell apoptosis of gastric carcinoma cell by Paeoniflorin and 5-FU was estimated by flow cytometry. Results：Paeoniflorin dramaticly inhibitied NF-κB activity in nuclear and cytoplasm. The inhibition pattern of NF-κB exhibited in a time and dose dependence,which was confirmed by Western Blot and ELISA. Moreover,Paeoniflorin had a promotion on cell apoptosis induced by 5-FU. Conclusion：Paeoniflorin could inhibit NF-κB activity in nuclear and cytoplasm of SGC-7901 ceils, and enhance cellular apoptosis of gastric carcinoma.