生长抑素或肠血管活性多肽调节大鼠肠淋巴细胞归巢机制初探

Preliminary study of somatostatin or vasoactive intestinal peptide regulating intestinal lymphocytes homing

目的:观察生长抑素(SST)或肠血管活性多肽(VIP)对大鼠肠淋巴细胞归巢肠相关淋巴组织的影响并初步探讨其作用机制。方法:VIP或SST体外孵育肠淋巴细胞,51Cr标记细胞后回输入大鼠体内,测定51Cr-细胞在肠相关淋巴组织(GALT)的数量。RT-PCR测定肠淋巴细胞SSTR-1～5和VIPR-1～2mRNA的表达。结果:经SST或VIP孵育的淋巴细胞在Peyer淋巴结数量较对照组减少,其在小肠弥散淋巴组织的数量较对照组无显著改变。肠淋巴细胞表达SSTR3和VIPR2基因。结论:生理状态下,SST或VIP抑制肠循环淋巴细胞归巢GALT,降低肠黏膜免疫功能,这一作用可能是通过SSTR-3和VIPR-2实现的。

Objective：To observe the influence of Somatostation （SST） or Vasoactive Intestinal Peptides （VIP） on intestinal lymphocytes homing to Gut-associated lymphoid tissues （GALT） and to primarily explore its possible mechanisms. Methods：Intestinal lymphoctes were collected from rats＇ intestinal lymphatics. After incubated with VIP or SST,they were labeled with ^51Cr and then infused into veins of rats. ^51Cr-lymphocytes in GALT were countered with γ counter 1 h later. And then the mRNA expression of SSTR-1-5,VIPR-1-2 on the lymphocyes were measured by RT-PCR. Results：The distribution of ^51Cr-lymphocytes treated with VIP or SST in Peyer＇s Patches were significantly less than that in control group, and no significant changes between diffusive lymphatic tissue of small intestinal and control group were observed. The rats＇ intestinal, lymphocytes apparently expressed the mRNA of SSTR-3 and VIPR-2. Conclusion：Under physical state,VIP or SST suppresses intestinal lymphocytes homing to Peyer＇ patches,which may be directly mediated by SSTR-3 or VIPR-2 on the intestine lymphocytes.