缺血预处理改善骨骼肌功能的研究

A study on the effect of ischemic precondition on skeletal muscle function

目的：探讨缺血预处理法改善缺血骨骼肌功能的临床价值。方法：用ＳＤ大鼠１２只，以右后肢为动物实验模型。分为缺血组（对照组，鼠６只），即缺血４小时后再灌注１小时的方法；缺血预处理组（实验组，鼠６只），缺血过程同对照组，但在缺血前预先经过２次缺血５分钟、再灌注１０分钟的处理。实验时，分别于缺血前、缺血１、４小时及再灌注１小时时，测定两组实验侧肢体腓肠肌最大肌张力的变化。实验结束后分别测量血ＭＤＡ、ＣＰＫ及大鼠右后肢９９ｍＴｃ亚甲基二磷酸计数。结果：实验组最大肌张力的变化（缺血４小时、再灌注１小时时）较对照组有明显改善；血ＭＤＡ、ＣＰＫ及肌肉９９ｍＴｃ亚甲基二磷酸较对照组显著降低。结论：缺血预处理不仅能改善骨骼肌的缺血耐受性。

Objective: To consider the clinical value of ischemic precondition in improving the functional recovery and reducing necrosis of skeletal muscle after severe ischemia. Methods:12 SD rats were randomly divided into two groups(control and precondition group). Ischemic model of the hind limb was set up. The isometric twitch contractile force of the right gastrocnemius was measured as a parameter indicating the muscle functional status. The degree of muscle damage was tested by the changes of MDA,CPK in blood sample and 99m Tc DMP counts of muscle. Results:There was an obvious increment of muscle contractile force in the experimental group at four hours of ischemia and one hour of reperfusion. The MDA and CPK level in blood and 99m Tc DMP count in muscle were significantly lower in the experimental group than in control. Conclusion:Precondition not only can increase the ischemia resistibility of skeletal muscle,but can also improve its functional recovery.