摘要
为了解在实际检测条件下，混合血清法筛检丙型肝炎抗体的效果，对１８７５例献血员进行了前瞻性研究。在单个血清检测结果未知的条件下，将每５个血清混合，使用酶免疫法检测丙型肝炎抗体。在检测中保持混合血清中每个标本的稀释度与单个血清检验法相同。结果显示：本次抗－ＨＣＶ筛检的血清阳性率为２．２４％，以单个血清检验为对照，混合血清法的灵敏度为１００％，特异度为９９．２％；阴性混合标本的ＯＤ／ＣＯ值呈正偏态分布，当混合标本中含有高ＯＤ值阴性标本时，可产生假阳性结果。收益分析发现：应用混合血清法筛检抗－ＨＣＶ可节省６９．３３％的检测费用。
A prospective survey of 1875 blood donors was carried out to determine the effect of the use of pooled sera to screen antibody to hepatitis C virus under real conditions.In the absence of knowledge regarding the individual test results,sera were pooled into groups of five.To guarantee the sensitivity of the test,we made the dilution and final volume in each pooled sera test the same as those for an individual serum test recommended by the test kit manufacturer.The results showed that taking individual sera as the control,the sensitivity of serum pooling strategy was 100%,but if the components of the positive pools were not separately tested,the specificity would be 99 2%.Analysis of cost effectiveness revealed that the serum pooling strategy reduced the cost by 69 33%.A strategy of pools of 5 was recommended as a cost effective approach to detect the antibody to hepatitis C virus by enzyme immunoassay in mass screening.
出处
《现代预防医学》
CAS
1997年第2期135-137,共3页
Modern Preventive Medicine
基金
江苏省卫生厅资助
