内异康复栓对子宫内膜异位症大鼠异位和在位内膜细胞色素C和生存素表达的影响

Effect of Neiyi Kangfu Suppository on Expressions of Cytochrome C and Survivin in Ectopic and Eutopic Endometrium in Rats with Endometriosis

目的探讨内异康复栓直肠给药对子宫内膜异位症(endometriosis,EMT)模型大鼠异位和在位内膜细胞色素C(cytochrome C,cytC)、生存素(Survivin)的不同影响。方法将EMT大鼠随机分为内异康复栓高、低剂量组,丹那唑组,丹莪妇康煎膏组,模型组和空白组,采用免疫组化SP法检测cytC、Survivin的表达。结果(1)内异康复栓高、低剂量组异位内膜cytC(IOD值)表达(分别为6.08±0.35、6.23±0.35)均显著增加,与模型组(5.07±0.70)及丹莪妇康煎膏组(5.98±1.02)比较,差异均有统计学意义(P<0.05,P<0.01);内异康复栓高、低剂量组Survivin(IOD值)表达(分别为5.73±0.93、5.62±0.93)显著降低,与模型组(6.01±1.16)比较,差异均有统计学意义(P<0.05,P<0.01)。(2)内异康复栓高、低剂量组能显著上调在位内膜下降的cytC水平,与模型组比较,差异有统计学意义(P<0.05);内异康复检高、低剂量组在位内膜Survivin的表达,与模型组和空白组比较,差异无统计学意义(P>0.05)。结论内异康复栓可能通过增加异位内膜cytC的表达并降低Survivin的表达,起到诱导凋亡的作用,而对在位内膜可上调cytC表达,对Sur-vivin表达无明显影响。

Objective To explore the different effects of reatal application of Neiyi Kangfu Suppository （NYKFS） on the expressions of cytochrome C （ Cyt C） and Survivin in ectopic and eutopic endometrium in endometriosis （EMT） model rats. Methods EMT rats were randomly divided into 6 groups, the high- and low-dose NYKFS groups, the Danazol Capsule （DN） group, the Dan＇e Fukang Soft Extract （DFE） group, the model group and the blank group. The expressions of Cyt C and Survivin were measured using immunohistochemical SP staining method. Results （1） After treatment, the IOD value of Cyt C in ectopic endometrium significantly increased in the high-dose and low-dose NYKFS groups respectively to 6.08±0. 35 and 6. 23±0. 35, which was significantly higher than that in the model group （5.07 ± 0. 70） and DFE group （5.98 _± 1.02） respectively （ P 〈 0. 05, P 〈 0. 01 ） ; while those of Survivin in ectopic endometrium was significantly decreased to 5.73 ± 0. 93 and 5.62 ± 0. 93 and was significantly lower than that in the model group （6. 01 _± 1.16, P 〈0. 05 or P 〈0. 01 ）. （2） The lowered eutopic endometrial level of Cyt C in EMT rats after treatment was significantly higher in the two NYKFS groups than that in the model group （ P 〈 0. 05 ） ; while that of eutopic endometrial Survivin was insignificantly different between the NYKFS groups and the model group or the blank group （ P 〉 0. 05 ）. Conclusion NYKFS plays its role in inducing apoptosis by increasing the expression of Cyt C and decreasing the expression of Survivin in ectopic endometrium, but it up-regulates expression of Cyt C, has no obvious effect on Survivin expression in eutopic endometrium.