尿道不全梗阻致大鼠尿液返流性前列腺炎模型

Intraprostatic Urinary Reflux Associated Prostatitis Caused by Partial Urethral Obstruction in the Rat Model

目的:用手术方法建立大鼠尿道不全梗阻致尿液返流至前列腺内,探讨慢性前列腺炎/慢性骨盆疼痛综合征(CP/CPPS)的发病机制。方法:54只雄性SD大鼠随机分为实验组(n=30)和假手术组(n=24)。实验组:严格参照Shinsuke Takechi手术方法行大鼠阴茎根部不全结扎,形成尿道不全梗阻,假手术组作为对照,在造模3d解除梗阻后第1、3、7d观察前列腺形态并取材行光镜观察,免疫组化方法检测环氧化酶-2(COX-2)的表达。结果:①实验组在尿道不全梗阻3d解除梗阻后在第1、3、7d前列腺出现可见的炎症变化,且随时间延长炎症逐渐减轻;而假手术组为正常前列腺组织。②免疫组织化学染色显示实验组前列腺内COX-2染色较假手术组明显增强(P<0.05);随着时间延长,COX-2染色加深(P<0.05)。结论:该试验提供了尿液返流性前列腺炎动物模型;前列腺内COX-2在尿液刺激后表达增强可能与CP/CPPS患者的疼痛不适症状有着密切的关系。

Objective： To investigate the pathogenesis of chronic prostatitis / chronic pelvic pain syndrome （ CP / CPPS） by constructing the rat model of intraprostatic urinary reflux associated prostatitis caused by partial urethral obstruction. Methods ： Fifty-four SD male rats were divided into an experiment group （ n = 30） and a partial urethral obstruction （PUO） sham operation group （ n = 24）. Shinsuke Takechi＇s surgical method was adopted to achieve PUO and induce intraprostatic urinary reflux in the experiment group. While in the sham operation control group, the prostates were harvested at 1,3 and 7 days after release from 3-day PUO, their morphological changes observed with the light microscope and the expression of cyclooxygenase-2 （ COX-2 ） examined by immunohistochemistry. Results ： Inflammation was observed in the prostate of the experiment group at 1, 3 and 7 days after release from PUO and alleviated with the passing of time, while the control group remained normal. The expression of COX-2 in the prostate was significantly higher in the experiment group than in the control （ P 〈 0.05 ） and the staining of COX-2 became stronger with the lapse of time （ P 〈 0.05 ）. Conclusion： An animal model of intraprostatic urinary reflux associated prostatitis was constructed. The up-regulated expression of COX-2 induced by intraprostatic urinary reflux may be closely related with the development of CP/CPPS.