金黄地鼠前列腺分泌蛋白对输卵管液糖蛋白的影响

Effect of Ventral Prostate Secretory Proteins on Oviductal Fluid Glycoproteins in Golden Hamsters

目的:在整体水平探讨前列腺分泌蛋白对金黄地鼠输卵管液中糖蛋白的影响。方法:金黄地鼠雄鼠依据手术方式的不同分为3组,分别为假手术组(SH)、附属性腺全去组(TX)和腹前列腺组(VP)(仅存前列腺)。收集与各手术组交配后不同时间点(交配后0.5、2、4、6h)的输卵管液(每一时间点及每一组,n=3),输卵管液蛋白经SDS-聚丙烯酰胺凝胶电泳,考马斯亮蓝或阿仙蓝染色分析,应用蛋白电泳印迹后与系列某一种特异性糖基专一性结合凝集素反应,分析糖蛋白的变化。结果:不同组雄鼠交配及不同时间点收集输卵管液蛋白电泳谱类似,约15条主要条带。凝集素结合谱示,麦胚凝集素(WGA)结合的相对分子质量(Mr)为32000、35500、47000、52000糖蛋白见于6h VP组输卵管液,而6h TX组可见Mr为81000、128000条带;与豌豆凝集素(PSA)结合Mr为37500、32000糖蛋白仅见于6h VP组,而6h TX组缺乏;仅6h VP组可见与双花扁豆凝集素(DBA)结合Mr为52000、47000糖蛋白,而6h TX组缺乏。而0.5、2、4h时间点收集的输卵管液各凝集素结合谱相似。结论:前列腺分泌蛋白可影响修饰交配6h后的输卵管液中含乙酰氨基葡萄糖、N-乙酰半乳糖胺/半乳糖和甘露糖糖链的糖蛋白。这些糖蛋白可能在胚胎的发育过程中起作用。

Objective： To investigate the effect of the secretory proteins of the ventral prostate on the glyeoproteins in the oviductal fluid of golden hamsters. Methods： Male golden hamsters were divided into four groups： sham operation （SH）, total removal of acces-sary sex glands （TX）, and retainment of the ventral prostate only （VP）. Oviductal fluid was collected from female hamsters at 0.5, 2, 4 and 6 h after mating with the males of different operated groups with or without ventral prostate. Glycoproteins were probed with a panel of lectins and their changes in the oviductal fluid were analyzed by Western blot. Results： The 47000, 52000, 81000 and 128 000 WGA-binding proteins were observed in the oviductal fluid of the 6 h TX group, the 32000, 35500, 47000 and 52000 WGA-binding glycoproteins noted in the 6 h VP group, the 47000, 68000, 95000 and 128000 pisum sativum agglutinin（PSA）-binding glycoproteins shown in the 6 h TX and VP groups, two extra 32000 and 37500 bands detected in the 6 h VP group, the 47000 and 52000 dolichos bifloms agglutinin （DBA）-binding glycoproteins present in the 6 h VP but absent in the 6 h TX group. Conclusion： Ventral prostate secretory proteins affect acetylglucosamine, N-acetylgalactosamine/galactose and mannose in the oviductal fluid collected 6 hours after mating. And these glycoproteins may play an important role in the development of embryos.