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寻常性银屑病皮损及非皮损中IL-23(p19/p40)和IL-12(p35/p40)mRNA的表达 被引量：4

The Expression of Interleukin-23(p19/p40) and Interleukin-12(p35/p40) in Lesional and Non-lesional Skin of Psoriasis
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摘要 目的检测寻常性银屑病患者皮损及非皮损处IL-23(p19/p40)和IL-12(p35/p40)mRNA表达,探讨其临床意义。方法采用逆转录-聚合酶链反应(RT-PCR)检测寻常性银屑病患者皮损、非皮损处及正常人皮肤中IL-23(p19/p40)和IL-12(p35/p40)mRNA的表达水平。结果寻常性银屑病患者皮损中IL-23p19及p40(IL-23/IL-12)mRNA的表达均高于非皮损组织和正常皮肤组织(P<0.05),且非皮损处高于正常对照组,差异有显著性(P<0.05);IL-12p35mRNA在银屑病皮损处、非皮损处和正常对照中表达水平差异无显著性(P>0.05)。结论在寻常性银屑病发病过程中,IL-23可能发挥较IL-12更重要的作用。 Objective To study the mRNA expression and function of interleukin-23 （p19/p40） and interleukin-12 （p35/ p40） in the lesional, non-lesional skin of psoriasis and of normal human skin. Methods The expressions of IL-23 （p19/p40） and IL-12 （p35/p40） mRNA were semi-quantitatively analyzed with reverse transcriptase-polymerase chain reaction （RT-PCR） in psoriatic lesions, non-lesions and normal skin tissues. Results The mRNA expression of IL-23p19 and p40 （IL-23/IL-12） was higher in the psoriatic lesion than that of non-lesional psoriatic and normal human skin （ P 〈 0.05 ）. The mRNA expression of IL- 23p19 and p40（IL-23/IL-12） was lower in the normal skin than that in non-lesional skin（P 〈 0.05）. There was no significant difference of the level of IL-12p35 mRNA in the psoriatic lesion ,non-lesional psoriatic skin and normal skin （P 〉 0.05 ）. Conclusion The results indicate that IL-23 might be more importance in the pathogenesis of psoriasis than IL-12.
作者 陈旭娥 谭志建 岳青 刘厚君 刘志香 李家文
机构地区 华中科技大学同济医学院附属协和医院皮肤科
出处 《中国皮肤性病学杂志》 CAS 北大核心 2008年第1期14-16,18,共4页 The Chinese Journal of Dermatovenereology
关键词 银屑病 IL-23 P19 p40(IL-23/IL-12) IL-12 P35 Psoriasis Interleukin-23 p19 p40（Interleukin-23/Interleukin -12） Interleukin -12 p35
分类号 R758.63 [医药卫生—皮肤病学与性病学]
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参考文献11

  • 1Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23 ,with biological activities similar as well as distinct from IL-12 [ J ]. Immunity ,2002,13 ( 5 ) : 715 - 725.
  • 2Cua DJ, Sherlock J, Chen Y, et al. Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain[ J]. Nature,2003,421 (6924) : 744 -748.
  • 3Robinson DS, OGarra A. Further checkpoints in Th1 development [ J ]. Immunity,2002,16 (6) :755 - 758.
  • 4Yano S,Komine M,Fujimoto M,et al. Interleukin 15 induces the signals of epidermal proliferation through ERK and PI3-kinase in a human epidermal keratinocyte cell line, HaCaT [ J ]. Biochem Biophys Res Commun,2003,301 ( 4 ) : 841 - 847.
  • 5Kopp T, Lenz P, Bello-Fernandez C, et al. IL-23 production by cosecretion of endogenous p19 and transgenic p40 in keratin 14/p40 transgenic mice : evidence for enhanced cutaneous immunity [ J ]. J Immunol,2003, 170( 11 ) :5438 -5444.
  • 6Wiekowski MT, Leach MW, Evans EW, et al. Ubiquitous transgenic expression of the IL-23 subunit p19 induces muhiorgan inflammation ,runting, infertility, and premature death [ J ]. J Immunol, 2001,166 ( 12 ) : 7563-7570.
  • 7Lee E ,Trepicchio WL, Oestreicher JL,et al. Increased expression of interleukin 23 p19 and p40 in lesional skin of patients with psoriasis vulgaffs[ J]. J Exp Med,2004,199( 1 ) : 125 - 130.
  • 8Piskin G, Tursen U, Sylva-Steenland RM, et al. Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers - IL-12,IL-18 and IL-23[ J]. Exp Dermatol,2004,13(12) : 764-772.
  • 9Chamian F, Lowes MA, Lin SL,et al. Alefacept reduces infiltrating T cells,activated dendritic cells, and inflammatory genes in psoriasis vulgaffs [ J ]. Proc Natl Acad Sci USA,2005,102 (6) :2075 - 2080.
  • 10李家文,李东升,刘厚君.寻常性银屑病患者皮损白介素17、干扰素γ及巨噬细胞炎性蛋白3αmRNA的表达[J].中华皮肤科杂志,2004,37(3):150-152. 被引量：8

二级参考文献18

  • 1Teranishi Y, Mizutani H, Murata M, et al. Increased spontaneous production of IL-8 in peripheral blood monocytes from the psoriatic patient: relation to focal infection and response to treatments[J]. J Dermatol Sci, 1995, 10(1): 8-15.
  • 2Deleuran M, Buhl L, Ellingsen T, et al. Localization of monocyte chemotactic and activating factor (MCAF/MCP-1) in psoriasis[J].J Dermatol Sci, 1996, 13(3): 228-236.
  • 3Rossi DL, Vicari AP, Franz-Bacon K, et al. Identification through bioinformatics of two new macrophage proinflammatory human chemokines: MIP-3alpha and MIP-3beta[J]. J Immunol, 1997 ,158(3): 1033-1036.
  • 4Tanaka Y, Imai T, Baba M, et al. Selective expression of liver and activation-regulated chemokine (LARC) in intestinal epithelium in mice and humans[J]. Eur J Immunol, 1999, 29(2): 633-642.
  • 5Dieu-Nosjean MC, Massacrier C, Homey B, et al. Macrophage inflammatory protein 3alpha is expressed at inflamed epithelial surfaces and is the most potent chemokine known in attracting Langerhans cell precursors[J]. J Exp Med, 2000, 192(5): 705-718.
  • 6Uyemura K, Yamamura M, Fivenson DF, et al. The cytokine network in lesional and lesion-free psoriatic skin is characterized by a T-helper type 1 cell-mediated response [J]. J Invest Dermatol, 1993, 101(5): 701-705.
  • 7Schon MP. Animal models of psoriasis - what can we learn from them?[J]. J Invest Dermatol, 1999, 112(4): 405-410.
  • 8Homey B, Dieu-Nosjean MC, Wiesenborn A, et al. Up-regulation of macrophage inflammatory protein-3 alpha/CCL20 and CC chemokine receptor 6 in psoriasis[J]. J Immunol, 2000, 164(12):6621-6632.
  • 9Chabaud M, Page G, Miossec P. Enhancing effect of IL-1, IL17, and TNF-alpha on macrophage inflammatory protein-3alpha production in rheumatoid arthritis: regulation by soluble receptors and Th2 cytokines[J]. J Immunol, 2001, 167(10): 6015-6020.
  • 10Albanesi C, Scarponi C, Cavani A, et al. Interleukin-17 is produced by both Th1 and Th2 lymphocytes, and modulates interferon-gamma- and interleukin-4-induced activation of human keratinocytes[J]. J Invest Dermatol, 2000, 115(1): 81-87.

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中国皮肤性病学杂志
2008年 第1期

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