应用组织芯片技术观察曝光皮肤中Ki67蛋白的表达

Ki67 expressions detected by tissue microarrays in sun-exposed skin

目的:比较曝光皮肤与非曝光部位皮肤组织的Ki67的表达情况。方法:使用组织芯片技术用免疫组化链霉亲和素-生物素法(Streptavidin Perroxidase,SP)进行检测。结果:在33例曝光皮肤中阳性16例(48.5%),阴性17例(51.5%),在18例非曝光皮肤中阳性17例(94.4%)(χ2=86.5,P<0.05)。同组中随着年龄增加,Ki67蛋白表达有波动;皱纹重较皱纹轻表达减少。银屑病5例,扁平苔藓5例均为强阳性,湿疹5例为中等强度阳性,脂溢性角化病2例强阳性。曝光皮肤组织Ki67表达较非曝光部位皮肤组织的Ki67蛋白表达减少。银屑病、扁平苔藓、脂溢性角化病Ki67表达与正常皮肤进行比较有明显增强。阳性表达分布于细胞核,位于基底细胞层及表皮全层。结论:(1)日光对细胞增殖因子的表达起抑制作用。(2)增殖性皮肤病及良性肿瘤细胞增殖能力强于炎性皮肤病,炎性皮肤病细胞增殖能力较正常组织强,正常皮肤组织细胞增殖能力较光曝晒组织强。

Objective： To compare the expression of Ki67 between sun- exposed and non- sun- exposed skin. Methods： The expression of Ki67 was measured by tissue microarray. Thirty- three specimens of sun- exposed skin, 18 specimens of non- sun- exposed skin, 5 specimens of psoriasis, 5 specimens of lichen planus, 5 specimens of eczema and 2 specimens of seborrheic keratosis were detected by immunohistechemistrical Streptavidin - Peroxidase method. Mouse antihuman monoclonal antibody was used. Results： Expressions of Ki67 were positive in 16 （48.9%） of 33 sun - exposed skin specimens, 17 （94.4%） of 18 cases in non - sun - exposed skin specimens （ P 〈 0.05）. All other specimens were positive. Psoriasis staining of Ki67 was located in nucleus and expressed in whole layer of epidermis. Conclusion： The expression of Ki67 in sun- exposed skin is less than that in non- sun exposed skin. These results indicate that sun exposure can decrease the proliferation of human skin and accelerate photoaging.