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重组核心蛋白聚糖转染对HepG2细胞周期、凋亡以及P21表达的影响 被引量：2

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摘要 目的将已构建完成的分泌型核心蛋白聚糖(DCN)真核表达载体转染到HepG2中并检测其表达同时研究其抗肿瘤作用的机制。方法脂质体介导分泌型DCN真核表达载体转染HepG2细胞,经G418筛选建立稳定转染的细胞株,采用RT-PCR、免疫组化检测其表达。MTT检测细胞增殖活力,流式细胞仪分析细胞周期,RT-PCR方法检测P21WAF1/CIP1mRNA表达情况。结果RT-PCR可见转染组细胞DCN mRNA表达明显增多,免疫组化可见转染组细胞DCN蛋白表达明显增高。细胞生长曲线显示转染组细胞生长缓慢;G1期细胞显著增多;P21WAF1/CIP1mRNA表达增高。结论本研究成功建立稳定转染DCN的HepG2细胞株,证实DCN通过阻滞细胞周期、诱导细胞凋亡和提高P21WAF1/CIP1蛋白抑制HepG2的生长。
作者 张玉成 王雅丽 杜珍武 赵虹 吕俊峰 张桂珍
机构地区 吉林大学中日联谊医院中心实验室 吉林大学第二临床医院放射线科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2008年第3期241-243,共3页 Chinese Journal of Gerontology
基金 吉林省发展与改革委员会计划资助项目〔2006〕1550号
关键词 核心蛋白聚糖 P21^WAF1/CIP1 细胞周期 转染 HEPG2
分类号 R329.28 [医药卫生—人体解剖和组织胚胎学]
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参考文献12

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同被引文献21

  • 1Stander M, Naumann U, Dumitrescu L,et al. Decorin gene transfer-mediated suppression of TGF-beta synthesis abrogates experimental malignant glioma growth in vivo[J]. Gene Ther, 1998 ;5 (9) :1187-94.
  • 2Reed CC, Gauldie J, Iozzo RV. Suppression of tumorigenicity by adenovirus-mediated gene transfer of decorin [J]. Oncogene, 2002 ; 21 ( 23 ): 3688- 95.
  • 3Iozzo RV. The family of the small leucine-rich proteoglycans:key regulators of matrix assembly and cellular growth [ J ]. Crit Rev Biochem Mol Biol,1997 ;32(2) :141-7.
  • 4Santra M, Eichstetter I, Iozzo RV. An anti-oncogenic role for decorin. Down-regulation of ErbB2 leads to growth suppression and cytodifferentiation of mammary carcinoma ceils [J]. J Biol Chem, 2000 ; 275 ( 45 ) : 35153-61.
  • 5Silvia G,Daniela GS, Heath J,et al. An antimetastatic role for decorin in breast cancer[J]. Am J Pathol,2008 ; 173 (3) :844-55.
  • 6Shintani K, Matsumine A, Kusuzaki K, et al. Decorin suppresses lung metastases of murine osteosarcoma[J]. Oncol Rep,2008 ; 19 (6) :1533-9.
  • 7Tralhao JG, Schaefer L, Micegova M,et al. In vivo selective and distant killing of cancer cells using adenovirus-mediated decorin gene transfer[J]. FASEB J,2003 ;17(3) :464-6.
  • 8Matsumine A, Shintani K, Kusuzaki K, et al. Expression of decorin, a small leucine-rich proteoglycan, as a prognostic factor in soft tissue tumors[J]. J Surg Oncol,2007 ;96 ( 5 ) :411-8.
  • 9Stander M, Naumann U, Wick W, et al. Transforming growth factor-beta and p-21 :multiple molecular targets of decorin-mediated suppression of neoplastic growth [J]. Cell Tissue Res, 1999 ;296 ( 2 ) :221-7.
  • 10Daniela GS, Silvia G, Christopher A, et al. Decorin protein core inhibits in vivo cancer growth and metabolism by hindering epidermal growth factor receptor function and triggering apoptosis via easpase-3 activation [J]. J Biol Chem,2006 ;281 : 26408 -18.

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中国老年学杂志
2008年 第3期

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