EMS1蛋白在胃癌组织中的表达及临床意义

Expression and Clinical Significance of EMS1 Gene in Gastric Carcinoma

背景与目的:研究表明EMS1(chromosome eleven,band q13,mammarytumour and squamous cell carcinoma-associated gene1)与多种恶性肿瘤的发生、发展及转移相关。本研究旨在探讨EMS1蛋白在胃癌、胃上皮内瘤变和正常胃粘膜组织中的表达。方法:采用免疫组化SP法,分别检测20例正常胃粘膜、38例胃上皮内瘤变和146例胃癌组织中EMS1蛋白的表达。结果:EMS1蛋白表达定位于细胞浆;正常胃粘膜EMS1蛋白阳性率20.0%(4/20),胃上皮内瘤变中阳性率68.4%(26/38),胃癌中阳性率89.7%(131/146);EMS1蛋白在胃癌中的表达高于胃上皮内瘤变及正常胃粘膜(P<0.001),胃上皮内瘤变与正常胃粘膜差异亦具有显著性(P<0.001)。早期胃癌阳性率为60.9%(14/23),低于进展期胃癌的95.1%(117/123),差异有统计学意义(P<0.001);伴淋巴结转移组EMS1蛋白阳性率为96.8%(91/94),与无淋巴结转移组的77.0%(40/52)相比差异有显著性(P<0.001);而与患者性别、年龄以及肿瘤大小、分化无关。结论:EMS1蛋白高表达与胃癌发生、淋巴结转移及临床分期有关。

BACKGROUND ＆ OBJECTIVE： EMS1 （chromosome eleven, band q13, mammary tumor and squamous cell carcinoma-associated gene 1 ） is correlated to the genesis, progression, invasion and metastasis of some malignancies. This study was to investigate the expression of EMS1 protein in gastric carcinoma, and to explore its correlation to the carcinogenesis of gastric carcinoma. METHODS： The expression of EMS1 protein in 20 specimens of normal gastric mucosa, 38 specimens of intraepithelial neoplasia, and 146 specimens of gastric carcinoma was detected by immunohistochemistry. RESULTS： EMS1 protein was expressed in cytoplasm. The positive rate of EMS1 protein was significantly higher in gastric carcinoma than in intraepithelial neoplasia and normal gastric mucosa （89.7% vs. 68.4% and 20.0%, P〈0.001）, and significantly higher in intraepithelial neoplasia than in normal gastric mucosa （P〈0.001）. The positive rate of EMS1 protein was significantly lower in the gastric carcinomas at early stages than in those at advanced stages （60.9% vs. 95.1%, P〈0.001）, and significantly higher in the gastric carcinomas with lymph node metastases than in those without lymph node metastases （96.8% vs. 77.0%, P〈0.001）. EMS1 protein expression had no correlations to sex, age, tumor differentiation and diameter. CONCLUSION： EMS1 protein expression is related to the carcinogenesis, lymph node metastasis and clinical stage of gastric carcinoma.