帕金森病大鼠苍白球神经元簇发放电及峰峰间期序列的研究

Analysis of the burst firing and sequence of ISI in the globus pallidus of Parkinson's disease model rats.

目的观察帕金森病大鼠模型苍白球神经元的电活动。方法30只大鼠注射6-羟基多巴胺(6-hydroxydopamine,6-OHDA)建立PD模型,并通过跑步机测试、注射阿朴吗啡诱发旋转和免疫组化检测黑质对模型进行评价;10只大鼠注射含0.2%抗坏血酸的人工脑脊液建立对照组。在立体定向仪引导下记录大鼠在PD病理及正常生理状态下GP神经元的自发放电活动。结果模型组大鼠中有13只行为学及病理学检测结果符合PD模型标准。电生理记录显示对照组大鼠GP神经元放电频率为(6.04±2.12)Hz,模型组大鼠GP神经元放电频率为(21.10±3.21)Hz(P=0.001)。模型组GP神经元簇发放电模式的比例术后4周为59%,术后8周为61%,而对照组GP神经元簇发放电模式的比例在术后4周和8周均为11%。模型组大鼠神经元放电的峰峰间期散点图在100ms以下有一分布密集条带。结论PD模型大鼠GP神经元较生理状态下放电频率明显增加,簇状放电模式比例增大,ISI序列发生明显变化。

Objective To explore the burst firing in the globus pallidus（GP） in a rat model of parkinson＇s disease （PD）. Methods The PD models were created using microinjection of 6-hydroxydopamine （6-OHDA） into the pars compacta of substantia nigra （SNc） and ventral tegmental area （VTA） in 30 Sprague-Dawley rats. Ten control animals underwent the same procdure with microinjection of ACSF. The electrophysiological recordings of the GP neuron were recorded in both OHDA-treated animals and controls. The ROTA-ROD test and immunohistochemical staining were used for behavioral and pathological evaluations. Results PD model was sucessfullycreated in thirteen rats as evidenced by behavioral and pathological evaluations. Electrophysiological record showed that the frequency of the burst firing was 6. 04±2. 12 Hz in the control animals and 21.10±3.21 Hz in PD rats （P =0. 001）, respectively, （P =0. OOl）. Burst firing rate was 59% at 4 weeks and 61% at 8 weeks post-lesion in PD rats, and both 11% in control rats. There was a close-set under lOOms in the return map of ISI. Conclusions The frequency of GP nearon firing and Burst firing increased and the sequence of ISI had a remarkable Chage in PD rats.