病毒性心肌病小鼠心肌线粒体能量代谢变化研究

Study of change of cardiac mitochondria energy metabolism in mice with viral cardiomyopathy

目的:观察实验性病毒性心肌病小鼠心肌线粒体能量代谢变化情况,探讨线粒体能量代谢变化在病毒性心肌病发病中的作用。方法:用柯萨奇病毒B3m反复增量感染BALB/C小鼠,建立心肌病动物模型,以荧光分光光度计比色法检测其细胞色素C氧化酶活力。结果:病毒性心肌病组小鼠线粒体氧化磷酸化标志酶细胞色素C氧化酶活力较对照组明显下降(P<0.05)。结论:心肌线粒体能量代谢障碍是病毒性心肌病的重要病理生理基础。

Objective： To study the role of change of mitochondria energy metabolism on pathogenesis of the viral cardiomyopthy by establishing the viral cardiomyopathy model in mouse. Methods： Balb/c mice were repeatedly increased infected with Coxsackievirus B3m （CVB3m） to establish experimental model of dilated cardiomyopathy. Fura-2/AM flourescein loading technique was used to evaluate the activity of cytochrome C oxidase. Results： The activity of cytochrome C oxidase which was a symbolic enzyme in oxidative phosphorylation was significantly decreased in comparison with that in normal control group （P〈0.01）. Conclusion： Severe cardiac mitochondria energy metabolism disorder is the important pathological and physiological foundation of viral cardiomyopathy.