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TIMP-4 mRNA在小鼠子宫中的表达和激素调节

Expression and Hormonal Regulation of Tissue Inhibitor of Metalloproteinase-4 in Mouse Uterus
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摘要 为研究TIMP-4 mRNA在子宫中的变化规律,采用半定量RT-PCR方法,观察了TIMP-4 mRNA在出生后小鼠子宫不同发育期、动情周期、妊娠早期以及假孕早期子宫中的表达和激素调节。结果发现,TIMP-4 mRNA在出生后不同发育期的小鼠子宫中、动情周期的任何时期、妊娠第1天以及假孕第1天的子宫中都不表达,但在妊娠和假孕第2天、第3天、第4天的子宫中都表达。以上结果提示,TIMP-4 mRNA在妊娠小鼠子宫中的表达可能不依赖存活胚胎的激活,而可能受母体妊娠分泌物的上调。 The tissue inhibitor of metalloproteinase-4 （TIMP-4） is the newest member of TIMP family and its function in the reproductive system remains unclear. In the present study, semi-quantitative RT-PCR was used to investigate its expression in mice uteri during postpartum period, the estrous cycle, early pregnancy and early psypregnancy. The results showed that TIMP-4 mRNA was not detected in mouse uterus during postpartum period and estrus cycle day 1 of pregnancy or day 1 of psypregnancy, but it could be detected only in the pregnancy day 2, 3,4 as well as the psypregnancy day 2, 3, 4, 5. These data suggested that TIMP-4 mRNA in uterus might be activated by materials derived from mother rather than embryo.
作者 卜淑敏 汪建红
机构地区 首都体育学院 新疆师范大学生命科学与化学学院
出处 《新疆师范大学学报（自然科学版）》 2008年第1期97-99,106,共4页 Journal of Xinjiang Normal University(Natural Sciences Edition)
基金 第35批博士后基金资助(2004035099)
关键词 TIMP-4 小鼠 子宫 表达和调节 TIMP-4 mice uterus expression and regulation
分类号 Q812.1 [生物学—生物工程]
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参考文献10

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二级参考文献10

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新疆师范大学学报（自然科学版）
2008年 第1期

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