氰戊菊酯对大鼠精子及生殖激素的影响

Effect of fenvalerate on sperm and sex hormone levels of male rats

目的探讨氰戊菊酯(Fen)对雄性大鼠精子计数、活力以及生殖激素的影响。方法成年雄性SD大鼠,分别以0、20、40、80mg/kg剂量的Fen连续灌胃染毒15和30d,按常规方法进行精子计数和精子活力检测,应用放射免疫法和化学发光免疫法测定大鼠血清卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)、雌二醇(E2)和睾丸匀浆中T、E2水平。结果Fen染毒15d时,与0mg/kg组相比,40mg/kg剂量组精子数量明显减少(P<0.01),20和40mg/kg组睾丸匀浆中T水平显著降低(P<0.01,P<0.05),血清LH、FSH水平随染毒剂量的增加而升高,且FSH水平和染毒剂量有显著的剂量-效应关系(P<0.05);Fen染毒至30d时,各组间精子数量差异不显著,与0mg/kg组相比,40mg/kg剂量组(a+b)级精子活力显著降低(P<0.05),血清LH、FSH水平随染毒剂量的增加而升高,但差异不显著。结论Fen对雄性大鼠有明显的生殖毒性作用,能够改变血清和睾丸中的生殖激素水平。

Objectives： To evaluate the effect of fenvalerate （Fen） on sperm counts, sperm motility and sex hormone levels of male rats. Methods： Different doses of Fen （0, 20, 40 mg/kg） were administered orally to the adult male SD rats for 15 days or 30 days, respectively. The sperm counts and sperm motility were evaluated, and the levels of serum follicle stimulating hormone （FSH）, luteinizing hormone （LH）, testosterone （T）, estradiol （E2） and testis homogenate T and E2 were determined by radioimmunoassay and chemiluminescent immunoassay. Results. Compared to those in the control （Fen 0 mg/kg group）, the sperm counts markedly decreased in the rats exposed to Fen of 40 mg/kg for 15 days （P〈0.01）. In addition, T levels in testis homogenates markedly decreased in the rats exposed to 20 and 40 mg/kg groups for 15 days （P〈0.01 and P〈0.05, respectively）. Serum levels of LH and FSH increased with the doses of Fen increased, with a significant dose-dependent increase in FSH levels （P〈0. 05）. After thirty days of exposure, the sperm counts showed no significant differences among three groups, but the grade （a＋b） sperm in 40 mg/kg group decreased significantly （P〈0.05）. LH and FSH levels in serum also increased with the doses of Fen increased, but not significantly （P〉0.05）. Conclusion： Fen has obvious reproductive toxicity on male rats, and can alter sex hormone levels in serum and testis homogenate.