哈尔滨地区肝炎患者血清中HBV DNA基因型分析

Genotyping for hepatitis B virus in sera from infected patients in Harbin

目的分析哈尔滨地区乙型肝炎患者HBV基因型的分布情况。方法采用基因型特异性引物巢式聚合酶链式反应法对96例肝炎患者血清中HBV DNA基因型进行分析。结果96例肝炎患者中92例HBV DNA检测阳性,其中80例患者属于C基因型,占87.0%。2例为B基因型,占2.2%。10例未检出型别。结论在哈尔滨地区HBV感染同时存在B和C基因型,以C基因型为主,且可能存在其他型别或发生基因突变。

Objective To genotype the hepatitis B virus （HBV） DNA in infected patients in Harbin.Methods HBV DNA in the serum samples of 96 hepatitis patients were tested using nested.PCR with genotype-specific primers. Results Ninty-two samples are HBV DNA positive among the 96 samples. Among the positive samples there were 80 C-type samples （87.0%） and 2 B-type （2.2%）.Ten samples could not be classified by the genotype-specific primers. Conclusion In Harbin there are HBV infection cases with genotype B and C multiplicity, and type C is predominant. There might be other genotypes or gene mutation.