大鼠脑缺血耐受与IL-17的表达

Cerebral ischemic tolerance and expression of IL-17 in rats

目的观察局灶性脑缺血预处理对IL-17表达的影响,探讨IL-17与脑缺血耐受的关系。方法利用线栓法建立局灶性脑缺血耐受的动物模型。通过脑梗死体积的测定、光镜下病理形态学变化和免疫组织化学染色及图像分析观察IL-17的表达。结果缺血组的脑梗死体积较耐受组明显增加。耐受组病理改变明显轻于缺血组。缺血组及耐受组比较IL-17阳性细胞平均面密度及阳性细胞平均灰度差异均有显著性(P<0.05)。IL-17的表达与脑梗死体积呈正相关(P<0.05)。结论局灶性缺血预处理能够诱导缺血耐受的产生,对随后的脑梗死有明显保护作用。IL-17可能与脑缺血耐受有关。

Objective To observe the effect of focal cerebral ischemic pretreatment on the expression of interleukin-17 （IL-17） and to investigate the relationship between IL-17 and cerebral ischemic tolerance in rats. Methods Middle cerebral artery occlusion（MCAO） was used to establish the model of focal cerebral ischemic tolerance. IL-17 expression and brain infarction volume, pathological morphological change were determined with immunohistochemistry. Results Infarction volumes in the ischemia group were significantly larger than those in the tolerance group. Significant differences in the mean area density and mean gray scale of IL-17 positive cells were found between the two groups （ P 〈 0.05） .The positive expression of IL-17 was correlated with the infarction volume （ P 〈 0.05）. Conclusion Focal cerebral ischemic pretreatment can induce ischemic tolerance and provide protective function for the following cerebral infarction obviously. IL-17 may be associated with the occurrence and development of cerebral ischemic tolerance.