ABO血型不合异基因造血干细胞移植后不同血型混合红细胞的分离鉴定

Isolation and identification of mixed erythrocytes with different blood groups from the patients following ABO-incompatible allogeneic haematopoietic stem cell transplantation

目的建立不同血型混合红细胞的分离鉴定方法，应用于ABO血型不合造血干细胞移植患者的混合红细胞分离鉴定及植活红细胞研究。方法应用红细胞血型抗原与相应抗体反应凝集，800×g和50×g分步离心，分离并收集凝集的和未凝集的红细胞进行计数和鉴定。结果18例ABO血型不合造血干细胞移植后均成功分离鉴定出植活红细胞和患者自身红细胞。初次分离鉴定出植活红细胞的时间为移植后第11天至第72天。植活红细胞数量随移植后时间改变。本分离方法的灵敏度为1％，准确度为100％，回收率为92．5％，重复性为100％。结论本方法可广泛用于不同血型混合红细胞的分离鉴定，为进一步研究干细胞移植患者2种不同红细胞变化的临床意义提供可靠的实验方法。

Objective To establish a new method for isolation and identification of mixed erythrocytes with different blood groups from the patients following ABO-incompatible allogeneic haematopoietie stem cell transplantation （allo-HSCT）, and investigate its application in post-transplantation survival erythrocytes. Methods The erythrocyte blood group antigens from the patiehts following ABO- incompatible allo-HSCT were agglutinated by the antibodies known, centrifuged at 800 x g and 50 x g to isolate agglutinated and unagglutinated erythrocytes respectively step by step. These erythrocytes collected were counted and identification respectively. Results The sensitivity, accuracy, recovery and reproducibility of the new method were 1%, 100%, 92.5% and 100%, respectively. The posttransplantation survival erythrocytes mixed with patient＇s erythrocytes from 18 patients following ABO- incompatible allo-HSCT were successfully isolated and identified by provided methods. It was the first time to identify the survival erythrocytes 11 th to 72 th day after allo-HSCT. The amount of the survival erythrocytes had been changing following post-transplantation periods. Conclusions The method can be widely employed in the isolation and identification of mixed erythrocytes with different blood groups. It provides reliable method to study the clinic significance of the changes of the survival erythrocytes from the patients following ABO-incompatible allo-HSCT.