脂氧合酶对唐菖蒲籽球形成和膨大的影响

Effects of Lipoxygenase on the Cormels Formation and Enlargement in Gladi-olus hybridus

为探讨脂氧合酶(LOX-1)对唐菖蒲籽球形成和膨大的影响,以‘Rose Supreme’和‘Ad- vanced Red’两个品种为试材,研究了籽球的生长发育与LOX-1活性,内源茉莉酸甲酯(MJ)、蔗糖、淀粉和纤维素含量变化之间的关系。结果表明,唐菖蒲叶片中检测不到LOX-1活性,匍匐茎中LOX-1活性和内源MJ含量最高,籽球数量较多的品种‘Rose Supreme’LOX-1活性和MJ含量显著高于‘Advanced Red’,说明LOX-1在唐菖蒲籽球形成和膨大过程中起着重要作用,其作用途径可能是通过影响茉莉酸类化合物(JAs)的生物合成对籽球的发生和生长进行调控。在唐菖蒲籽球生长发育过程中,新球和籽球中蔗糖、淀粉和纤维素含量都不同程度升高,并且与LOX-1活性呈正相关,说明蔗糖、淀粉和纤维素对唐菖蒲新球和籽球的形成和膨大起着重要作用。

To investigate the effects of lipoxygenase （LOX-1） in Gladiolus hybridus, with ‘ Rose Supreme＇ and‘ Advanced Red＇ on the cormels formation and enlargement as materials, LOX-1 activity, endogenous methyl jasmonate （MJ）, sucrose, starch and cellulose contents were examined during the cormels growth. The results showed that LOX-1 activity and endogenous MJ content in‘ Rose Supreme＇ were higher than those in ‘ Advanced Red＇ , with highest activities and contents in stolons, but not detected in leaves. It suggested that LOX-1 might regulate the development and growth of Gladiolus cormels through affecting the JAs biosynthesis cycles. During the cormels growth, the contents of sucrose, starch and cellulose, with positive correlations with LOX-1 activity, showed different increase in corm and cormels, which also suggested that sucrose, starch and cellulose played important roles in the formation and enlargement of Gladiolus corms and cormels.