黄瓜抗炭疽病相关基因AFLP标记的SCAR转换

Development of SCAR Marker Linked to Cucumber Anthracnose Resistance-related Gene from an AFLP Marker

将黄瓜抗炭疽病相关基因的一个共显性AFLP标记成功地转换成了简单实用的SCAR标记。对AFLP标记片段进行序列测定,根据序列特点设计了特异的SCAR引物,引物长18～21 bp。PCR结果表明,引物对(1)可以扩增出131 bp和125 bp两条带,引物对(2)可以扩增出178 bp和172 bp两条带,分别为抗炭疽病的特征带和感炭疽病的特征带。将该两个标记命名为SCEM131/125和SCEM178/172,两对引物在F_2单株和抗感病材料验证中符合率高达97.27%,可以用于黄瓜炭疽病的分子鉴定。

The co-dominant AFLP marker of anthracnose resistance-related gene of cucumber was converted into co-dominant SCAR marker successfully. After sequencing of the AFLP marker fragment, two pairs of primers were designed according to the nucleotide sequence. PCR results from cucumber genomic DNA showed the two primer pairs （ 1 and 2） could amplify two band types with the size of 131 bp/125 bp and 178 bp/172 bp, respectively. Both 131 bp band and 178 bp band were the character band of anthracnose resist- ance phenotype. These SCAR markers were designated as SCEM131/125 and SCEM178/172. Anthracnose resistance of F2 and some resistant and susceptible cucumber lines were tested by SCAR markers, and the re- suits indicated that the resistance tested was consisitent with their field performance by 97.27%, which further confirmed the feasibility of the SCAR markers in MAS for cucumber anthracnose.