一个中国水仙MADS-box基因的克隆与分析

Cloning and Sequence Analysis of a Flowering-related MADS-box Gene in Narcissus tazetta var.chinensis Roem.

采用RT-PCR技术从中国水仙(Narcissus tazetta var.chinensis Roem.)幼嫩花蕾中分离到一个新的水仙花发育相关MADS-box基因,命名为NTMADS1(GenBank登录号:EF421828)。该基因长879bp,编码区共编码230个氨基酸,具有典型的植物MADS-box基因结构,由MADS区、I区、K区和C末端组成。序列分析结果表明,NTMADS1编码的蛋白与其它植物的MADS-box蛋白有着较高的一致性,其中与扫状天门冬(Asparagus virgatus Baker)的AVAG1的一致性高达89%。系统进化树分析表明NTMADS1属于AG亚族。

MADS-box genes play important role in various plant development processes, especially in the development of floral organs. The flowering-related MADS-box gene, named as NTMADS1 （ GenBank accession number： EF421828）, was cloned from the first strand of Narcissus tazetta var. chinensis Roem. cDNA through RT-PCR using primers designed according to the MADS-box gene family conserved region. The fragment was 879 bp long and contained an open reading frame of 693 bp coding a polypeptide of 230 amino acids which had a typical MADS-box domain. Homology analysis showed that the deduced NTMADS1 protein was highly homologous to other MADS-box proteins from different species, especially with Asparagus virgatus Baker to 89%. Phylogenetic tree analysis also indicated that NTMADS1 belongs to AG subfamily.