摘要
以山西单雌、山东单雌、东北单雌、法国单雌和转1号标雌等5个蓖麻品种为研究材料,采用改良的CTAB-DNA提取法,从蓖麻叶片中提取基因组DNA;并通过对RAPD-PCR反应体系中dNTP、引物、模板DNA及TaqDNA聚合酶用量的梯度处理,运用多因素试验的方法进行优化。试验结果表明,该反应体系的体积为25μL时,各反应物适宜用量分别为:dNTP0.4mmol/L,随机引物0.2μmol/L,模板DNA120ng,Taq DNA聚合酶2.5U。在此反应体系下,扩增效果清晰、稳定。
To establish an optimal system of RAPD marker of castor haplometrotic traits, five oil castor haplometrotic cuhivars were investigated in this research. Leaf genomic DNA was extracted using a modified CTAB method. The RAPD reaction system was optimized by fine-tuning of concentrations template DNA, dNTP, random primers and Taq DNA polymerase. 25μL of the optimal system for RAPD-PCR consisted of 120ng of template DNA, 1.0×10^-2mmol dNTP, 1.0 ×10^-5 mmol random primers and 2. 5U of Taq DNA polymerase.
出处
《作物杂志》
CAS
CSCD
北大核心
2008年第1期31-33,共3页
Crops
基金
内蒙古自然科学基金(200607010312)
关键词
蓖麻
单雌
RAPD
优化
Castor
Haplometrotic cultivars
RAPD
Optimal system
