摘要
研究了外源ABA和乙烯利胁迫处理对斑茅1-氨基环丙烷-1-羧酸氧化酶基因(ACO基因)表达情况的影响。在项目组克隆斑茅ACO基因的基础上,应用实时荧光PCR技术分析斑茅ACO基因在A-BA、乙烯利胁迫下的表达。结果表明,斑茅ACO基因在ABA的胁迫下,在3h有微弱上调表达,而6h时明显抑制,其后表达趋向平缓;在乙烯利的胁迫下,ACO基因在前期受抑制,其后呈明显上调表达,在24h后ACO基因的表达趋向平缓。斑茅ACO基因的表达受乙烯利诱导,而不受外源ABA诱导。
In order to study the molecular mechanism of A CO gene from Erianthus arundinaceum under exogenous ABA and ethephon stress. In this paper, based on the cloning of A CO gene in Erianthus arundi- naceus, the expression of A CO gene in Erianthus arundinaceus under ABA and ethephon treatments was investigated by Real-time fluorescence PCR. The results showed that the expression of A CO gene was slightly up-regulated at the beginning and continuous inhibited, then became normal by ABA stress . Under the treatment of ethephon, the expression of A CO gene was prohibited at the earlier stage along with the subsequent continuous more obvious up-regulation. The results showed that the expression of A CO gene in Erianthus arundinaceus could be induced by ethephon, but not by ABA.
出处
《中国农学通报》
CSCD
2008年第2期48-52,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金"甘蔗抗旱系列基因的分离克隆及其芯片表达研究"(30370901)
教育部高等学校博士学科点专项科研基金项目(20040389009)资助
