摘要
目的建立测定注射用炎琥宁的含量的高效液相色谱方法。方法 采用Diamonsil^TM C18柱(4.6mm×200mm,5μm),甲醇-0.05mol·L^-1。磷酸二氢钾溶液[用磷酸调pH值至(2.5±0.05)](68:32)为流动相,流速:1.0mL·min^-1,检测波长为251nm。结果炎琥宁在0.04312~0.1724mg·mL^-1峰面积与其测定浓度呈良好线性关系(r=O.9999),平均回收率为99.9%,重复性试验RSD为0.49%(n=6)。结论本法准确、简便、快速,可用于注射用炎琥宁的含量测定。
Objective To establish an HPLC method to determinate potassium sodium dehydroandrandrographolide succinate. Methods The separation was performed on DiamonsilTM C18 column (4.6 mm×200 mm, 5 μm). The mobile phase was composed of methanol - 0.05 mol ~ L^-1 potassium dihydrogen phosphate solution -diethylamine [pH adjusted to (2.5±0.05) by H3PO4] (68 : 32) with the flow rate of 1.0 mL· min^-1 , and the detective wavelength was 251 nm. Results There was a good linearity within 0. 043 12~0. 172 4 mg ·mL^-1 of potassium sodium dehydroandran drographolide succinate (r=0. 999 9). The average recovery was 99.96%, and the RSD was 0.49% (n=6).Conclusion This method is accurate, simple and rapid. It is suitable for the quality control of potassium sodium de- hydroandrandrographolide succinate for injection.
出处
《中南药学》
CAS
2008年第1期65-67,共3页
Central South Pharmacy
关键词
注射用炎琥宁
高效液相色谱法
含量测定
potassium sodium dehydroandran drographolide succinate HPLC content determination