新疆产甘草品质评价及甘草多糖提取工艺研究

Quality Evaluation of Sinkiang Glycyrrhiza and Extraction of Glycyrrhiza Polysaccharide

本文通过测定样品中甘草酸和甘草多糖的含量,比较不同新疆产甘草切片品质;并通过单因素和正交实验研究甘草多糖的提取条件。结果表明,人工种植甘草毛草的甘草酸及甘草多糖含量分别为2.9%和5.0%,高于统草(甘草酸2.2%和甘草多糖3.5%),而野生甘草样品中,含量最高的仅有2.4%和3.3%;十年生野生甘草中甘草酸和甘草多糖含量均为2.4%,与五年生及3-5年生甘草根切片无显著性差异;甘草多糖的提取条件为:30倍料/液比,90℃提取0.5h,提取2次。

In this study, the characteristics of different Sinkiang Glycyrrhizas were compared by determining the contents of glycyrrhiza acid and glycyrrhiza polysaccharide. And the extraction conditions of glycyrrhiza polysaccharide were investigated via single factor and orthogonal experiments. The results showed that the side branches of planted Glycyrrhiza contained 2.9% glycyrrhiza acid and 5.0% glycyrrhiza polysaccharide, which were higher than those in the trunks （glycyrrhiza acid 2.2% and glycyrrhiza polysaccharide 3.5%）. In the wild glycyrrhiza, the highest contents of glycyrrhiza acid and glycyrrhiza polysaccharide were 2.4% and 3.3%, respectively. Ten years old wild glycyrrhiza contained 2.4% glycyrrhiza acid and glycyrrhiza polysaccharide. For the extraction of glycyrrhiza polysaccharide, the most suitable ratio of solid to liquid, extracting temperature, time and times were found to be 1：30,90℃, 0.5 h and twice, respectively.