摘要
采用高效液相色谱法测定酱油中5-羟甲基糠醛（5-HMF）的含量。样品经乙酸乙酯提取后，用C18色谱柱分离，以甲醇-水（体积比为5：95）为流动相，在UV284 nm波长条件下进行检测。该方法快速、准确，在0．5--20mg/L范围内线性相关系数为r^2=0．9992，平均回收率为82．4%--86．9％，相对标准偏差为3．2%--5.1％。方法的最低检测浓度为0．1mg／L。
A new method for the determination of 5-hydroxymethylfurfural by high performance liquid chromatography in soy was developed, After extracted by ethyl acetate, the samples were analyzed on a C18 column at 284 nm using a mixture of methanol and water （5：95, v/v） as the mobile phase. Within the linear range of 0.5-20.0 mg/L, the method was fast and accurate with the average recoveries rate and relative standard deviation of 82.4%-86.9 % and 3.2 %-5. 1%, respectively. The minimum determination limit was 0.1mg/L.
出处
《现代食品科技》
EI
CAS
2008年第2期188-190,共3页
Modern Food Science and Technology
关键词
酱油
5-羟甲基糠醛
高效液相色谱法
soy
5-hydroxymethylfurfural
high-performance liquid chromatographic（HPLC）
