辐照与去白细胞过滤单采血小板对血小板诸参数与部分细胞因子含量影响

Influence of radiation and leukocyte depletion on platelet parameters and cytokines in platelet concentrates

目的:了解应用去白细胞过滤器制备去白细胞单采血小板以及经γ射线辐照单采血小板血小板参数与血浆部分细胞因子含量变化情况。方法:应用Sysmex F-800血液分析仪检测血小板参数与应用ELISA法检测血浆IL-2、IL-6、INF-γ与TNF-α细胞因子。结果:去白细胞单采血小板与经γ射线辐照单采血小板悬液血小板包括:血小板数、血小板平均体积、血小板平均宽度、血小板压积以及血浆IL-2、IL-6、INF-γ与TNF-α含量与对照组相互比较差异均无统计学意义(P>0.05)。结论:单采血小板经去白细胞或经γ射线辐照后,不会影响血小板参数与血浆部分细胞因子含量。

Objective：To study the change of platelet parameters and cytokines in platelet concentrates which experienced leukocyte depletion or γ-radiation. method：Platelet parameters were tested by Sysmex-800 blood analyser and plasma IL-2,IL-6, IFN-γ and TNF-α levels were tested by ELISA. Result：After tested platelet parameters including PLT,MPV, PDW, PCT and the levels of IL-2,IL-6,IFN-γ and TNF-α showed no obvious differences among leukocyte depletion, γ-radiation, and control group（ P〉0.05）. Conclusion ： The ptatelet parameters and levels of cytokines in platelet concentrate will not be influenced after leukocyte depletion or γ-radiation.