摘要
利用掺杂聚乙烯醇的聚苯乙烯作粘结剂,制备成新型碳糊电极,该电极具有制作简单、导电性能良好和改进的表面吸附性能,可用于构建基于表面吸附固定生物组分的电化学免疫传感器。用转铁蛋白-转铁蛋白抗体作模式分析物,辣根过氧化物酶(HRP)作为酶标,建议的免疫传感器测定人转铁蛋白的线性关系是0.50~70.0μg/mL(相关系数为0.983),免疫传感器在使用后可通过简单的打磨抛光更新,表现出良好的重复使用性。
A new strategy to constructing amperometric immunosensor for transferrin assay based on improved carbon paste electrode (CPE) as sensing platform has been described. The CPE is prepared by employing polyvinyl alcohol-doped polystyrene as the novel adhesive. The obtained CPE has good conductivity and better surface -adsorption capability. A sequential competitive immunoassay format was performed on the surface of CPE by using transferrin and transferrin antibody as a model system. The assay comprised first loading of the transferrin antibody on surface of CPE, then blocked in BSA solution followed by a competitive incubation in the buffer containing the transferrin (analyte) and transferrin labeled with horseradish peroxidase (SjAg-HRP) and finally the amperometric detection. The feasibility of regenerating surface of CPE for consecutive assays is demonstrated by a simple polish after each determination. The dynamic concentration range for transferrin assay is 0.50 - 70.0 μ g/mL.
出处
《湖南工业大学学报》
2008年第1期99-103,共5页
Journal of Hunan University of Technology
基金
湖南省教育厅青年课题基金资助项目(05B075)
关键词
电化学免疫传感器
聚苯乙烯/聚乙烯醇
粘结剂
碳糊电极
electrochemical immunosensor
polyvinyl alcohol-doped polystyrene
adhesive
carbon paste electrode