豇豆胰蛋白酶抑制剂基因(cpti)及其在抗虫转基因作物中的应用

Cowpea Trypsin Inhibitor Gene (cpti) and its Application in Insect Resistance Transgenic plants

豇豆胰蛋白酶抑制剂(cowpea trypsin inhibitor,CpTI)来自豇豆的可食用部分,由于其具有抗虫谱广且昆虫不易对其产生耐受性的特点,cpti基因被作为抗虫植物基因工程一个重要的候选基因。目前,cpti基因已经被转到烟草、水稻、棉花和番茄等多种作物中。本文介绍了CpTI的特性、抗虫机理、基因工程研究进展,以及转cpti基因水稻的安全性研究,并对蛋白酶抑制剂的分类和安全性问题做了相关介绍。CpTI在分类上属于Bowmun-Birk家族,大量医学研究表明,该家族蛋白质对于癌症的预防和辅助治疗都有积极作用,对人体不存在任何危害。

Cowpea trypsin inhibitor （ CpTI ） is derived from the edible part of cowpea, cpti gene is an important candidate gene in plant genetic engineering for its broad insect resistance spectrum, and ability of delaying the insect resistance. At present, cpti gene has been transformed into tobacco, rice, cotton and tomato etc. This paper intro- duces the characteristics of CpTI, its insect resistance mechanism and research progress in gene engineering, including the research and safety evaluation of cpti transgenic rice. CpTI belongs to the Bowman-Birk family, which plays an active role in preventing cancer occurrence and as an assistant therapy, and is applied as an anticarcinogenic agent without any toxicity to human beings.