H5亚型血凝素基因的插入对重组LaSota毒株在组织中病毒分布的影响

The Impact of HA Gene of H5N1 Being Inserted into Recombinate Lasota Strain on the Distribution of Viral Antigen

为了研究外源基因H5亚型血凝素基因插入到NDV后对重组LaSota毒株在组织中对NDV病毒分布的影响,用构建的rL-F(M)-FHA和rL-FHA与亲本毒株重组LaSota野毒株(rLaSota)经滴鼻、点眼免疫SPF雏鸡,分别在免疫后1 d、3 d、5 d、10 d、15 d和18 d后采集病料(气管、肝脏,肺脏、肾脏、大脑、脾脏、腺胃、十二指肠),利用免疫组化染色方法检测病毒在机体内的分布。H5亚型血凝素基因的插入对NDV病毒分布、病毒在组织中出现时间有很大影响,而F基因突变株的变化也是影响病毒分布、病毒在组织中出现时间的主要因素。

In order to study the impact of HA gene of H5N1 on the viral antigen distribution, we inserted foreign gene （HA gene of H5N1 highly pathogenic avian influenza virus） between the M gene and P gene based on the F gene being exchanged rLaSota and rLaSota backbone, which were named as rL-F（M）-FHA and rL-FHA. Vaccination was given by eyedrop and intranasally instillation with rL-F（M）-FHA, rL- FHA and rlaSota to 4-day-old SPF chickens, after 1 d, 3 d, 5 d, 10 d, 15 d or 18 d, we collected the trachea, lung, liver, kidney, brain, spleen, proventriculus, duodenum. The distribution of viral antigen in tissues were detected by using immunohistochemical （ IHC ） staining. The results showed that HA gene of H5N1 being inserted rLaSota and F gene being exchanged had great influence to the distribution of viral antigen.