不同方法从大豆不同组织中提取基因组DNA效果的比较

Comparison of the Genomic DNA Extraction Methods From Soybean Different Tissues

从大豆组织中获得高质量和足够产量的基因组DNA,是进行大豆分子生物学研究的基础。为进行大豆基因组的PCR,RAPD,SSR等分子生物学研究,分别以大豆种子和其叶片为实验材料,采用改良的SDS法和CTAB法对大豆基因组DNA进行了提取。对DNA的提取效果,采用紫外分光光度检测、琼脂糖凝胶电泳分析及DNA的限制性内切酶图谱分析进行了综合比较。结果表明,在以叶片为材料时,SDS法和CTAB法的大豆基因组DNA的提取效果差别不大,SDS法稍好于CTAB法。在以大豆种子为材料时,SDS法的提取效果明显优于CTAB法,SDS法可从大豆种子中提取到能够充分满足各种分子操作的高质量和数量的大豆基因组DNA。

It is important to extract high quality and sufficient yield of genomic DNA for soybean molecular biological research. In order to study the soybean genome by PCR, RAPD, SSR etc. ,improved SDS and CTAB method were used to extract the soybean genomic DNA from the soybean seeds and leaves. The DNA extraction effect of two different methods was compared by ultraviolet spectrophotometry, agarose electroforesis and the restriction enzyme digestion. The results showed that the extraction effect of the SDS method and the CTAB method to soybean genomic DNA was not obviously different for soybean leaves. However,the extraction effect of SDS method is much better than CTAB method for soybean seeds. The conclusion is that the SDS method is able to extract high quality and quantity soybean genomic DNA, which is suitable for various molecular operations.