马尾松无性系种子园遗传结构的RAPD分析

Genetic Structure of Clonal Seed Orchard of Masson Pine by RAPD Technology

采用RAPD技术分析马尾松无性系种子园的遗传结构。结果表明：湖北京山马尾松无性系种子园具有较高的遗传多样性,其49个分离的RAPD位点的基因多样度（H）平均达0.3169,Shannon信息指数（I）平均达0.4813,其中多态位点百分数（P）为71.01%,与同工酶研究的马尾松天然群体的P值（68.06%～77.78%）十分接近。各种源间遗传距离的总体平均值为0.1074,群体间的遗传距离则以湖南和江西最小（0.0225）,其次是江西和浙江（0.0231）,广东和江西的遗传距离最大（0.2175）。遗传距离与空间距离之间的相关关系不显著,而与纬度之间的相关关系较显著。

An experiment was conducted to study the 123 plus trees from 8 provenances in clonal orchard of masson pine by RAPD technology. Result indicates that the clonal seed orchard of masson pine has higher genetic diversity. The average genetic diversity of the clonal seed orchard is 0. 316 9, the Shannon＇ s information index is 0. 481 3, and the percentage of polymorphic loci is 71.01%, which is similar to the result of natural populations studied by isozyme analyses（68.06% - 77.78% ）. The average genetic distance among populations is 0. 107 4. The smallest genetic distance is between Jiangxi Provenance and Hunan Provenance, the next is between Zhejiang Provenance and Jiangxi Provenance, and the largest one is between Guangdong Provenance and Jiangxi Provenance. There is no significant correlation between genetic distance and geographic distance, while the correlation between genetic distance and latitude is more significant.