固相萃取-高效液相色谱法测定水中痕量酚

Determination of Trace Phenols in Water by Solid Phase Extraction and High Performance Liquid Chromatography

研究了水中7种痕量酚类物质的前处理方法和采用高效液相色谱（HPLC）分析的色谱条件。用Waters Oasis HLB固相萃取小柱预分离和富集水中的酚，采用Agilent ZORBAX-C18分析柱进行色谱分离，以乙腈和0．1mol／L的磷酸作为流动相进行线性梯度洗脱，流速为1．5mL／min。各组分均在其最大紫外吸收波长下进行检测。结果表明，7种酚在13min之内能够很好地分离，回收率为80％～104％，相对标准偏差为2．4％～8．5％，色谱最低检测浓度达0．1～0．4mg／L，方法检出限为0．1～0．4μg／L。

The pretreatment methods of seven trace phenols in water and the conditions of using high performance liquid chromatography （HPLC） were studied. Phenols in water can be preseparated and enriched by solid phase extraction with Waters Oasis HLB cartridge, and separated on an Agilent ZORBAX-C18 column. The linear gradient elution with acetonitrile and 0.1 mol/L phosphoric acid as mobile phase was performed with flow velocity of 1.5 mL/min. The various components were tested at their maximum UV absorption wavelength. The seven phenols were separated completely within 13 min. The method recoveries range from 80% to 104%, and the RSD are 2.4% to 8.5%. The lowest HPLC detection concentrations are 0.1 to 0.4 mg/L, and the method detection limits are 0.1 to 0.4 μg/L.