石化废水处理系统微生物群落结构PCR-DGGE分析

Analysis of Microbial Community of Activated Sludge in Different Aeration Basins within Petrochemical Industry Wastewater Treatment System by PCR-DGGE

为提高石油化工污水厂厌氧—好氧(A/O)工艺净化效能,应用聚合酶链式反应—变性梯度凝胶电泳(PCR-DGGE)与常规技术相结合的方法分析了石化废水处理系统生化池中微生物群落结构变化与污染负荷和主要污染物降解效果变化的关系。结果表明:在每天2次,连续6天的监测中,待处理废水中COD负荷在424.92 mg/L和559.10 mg/L之间波动,NH3-N在63.60 mg/L和100.87 mg/L之间变化;工艺对COD和NH3-N的去除率较低,分别为69.42%和31.2%,且生化池各段对COD和NH3-N的去除率变化并非呈单一递减趋势,这与微生态细菌种类随污水浓度的变化呈非单一的递减趋势相一致;PCR-DGGE图谱中各处理单元的细菌条带数量变化很大,相似性系数最高为36.11%,最低为6.25%,微生物群落结构相似性很低。

To enhance the efficiency of A/O process for petrochemical wastewater treatment, the relationship between bacterial community structure and the pollutants loading/degrading rates are analyzed by the polymerase chain reaction and denaturing gradient gel electrophoresis （PCR-DGGE） technique. The results show： in the condition of COD loading between 424.92 mg/L and 559.10 mg/L and NH3-N between 63.60 mg/L and 100.87 mg/L, the removal rate of COD and NH3-N is low as 69.42% and 31.2% respectively. The removal rate of COD and NH3-N fluctuats with bacterial categories in the bioreactor. The profile of bacterial community in different units by PCR-DGGE show that the number of bacterium bands chang a lot and the highest similar coefficient is 36.11% and the lowest is 6.25 %, and that the similarity of bacterial community structure is extremely low. The experimental results indicate that the relationship between structure of bacterial community and its functions may be elucidated with the analysis of such variation.