葡萄籽原花青素提取物对膳食诱导肥胖小鼠氧化应激的影响

Effect of grape seed procyanidins extract on reducing oxidative stress in diet-induced obesity mice

目的观察强抗氧化剂葡萄籽原花青素提取物(grape seed procyandins extract,GSPE,低聚物>85%)对肥胖伴氧化应激小鼠抗氧化能力的影响。方法选用C57BL/6J小鼠,观察高、中、低剂量GSPE(400mg/kg·d-1、200mg/kg·d-1和100mg/kg·d-1)对膳食诱导肥胖(diet-inducedobesity,DIO)小鼠成模过程中体重、体脂、血清游离脂肪酸、血清肿瘤坏死因子α、肝谷胱甘肽过氧化物酶(GSH-Px)、肝过氧化物歧化酶(SOD)、肝总抗氧化能力(T-AOC)和丙二醛(MDA)的影响。结果GSPE能显著降低DIO小鼠的体重、体脂比,降低血清游离脂肪酸、血清肿瘤坏死因子α的水平,提高肝GSH-Px、SOD、T-AOC活性。结论GSPE有良好的预防DIO小鼠肥胖及其相伴的氧化应激的作用。

Objective Study the effect of Grape Seed Prucyanidins Extract （GSPE） on oxidative stress in diet-lnduced obesity mice. Methods Folly-eight male C57BL/6J mice were divided randomly into 6 groups according to their body weight： lower（100mg/kg·d^-1）, medium（200mg/kg·d^-1）, high（400mg/kg·d^-1） close GSPE group, ohesity group, TZD（3 mg/kg） group and control group. The mice in control group were fed with low fat （10kcal%） diet, and the other mice were fed with high fat （45kcal%） diet. In the control and obesity group, the mice were fed an oral gavage with water. In the GSPE/TZD group, the mice were given an oral gavage of GSPE/TZD in aqueous solution for 70d continuously. The weight, Lee＇s index, serum FFAs, serum TNF-a, hepatic T-AOC, hepatic SOD and GSH-Px activity of various groups were measured. Results GSPE can significantly reduce serum FFAs, increase hepatic T-AOC, SOD,GSH-Px activity of DIO mice. while the low and medium doses of GSPE can evidently prevent weight gain, adipose tissue mass increase in DIO mice. Conclusions The study indicates that GSPE is effective in up-regulating the antioxidant defense mechanism, preventing the weight gain and adipose tissue mass increase. GSPE in low and medium dose are better for preventing obesity induced hy high fat food.