牛蒡子提取物中牛蒡苷和牛蒡苷元的含量测定研究

Determination of Arctiin and Arctigenin in the Extract of Fructus Arctii

目的:建立牛蒡子提取物中牛蒡苷及其苷元的含量测定方法。方法:采用HPLC,以牛蒡苷、牛蒡苷元为含量测定指标,测定了牛蒡子提取物中牛蒡苷和牛蒡苷元的含量。色谱柱为KromasilC18100A(150mm×4.6mm,5μm)。流动相:甲醇-水(采用梯度洗脱的方式:0～7min,35%～45%甲醇;7～9min,45%～48%甲醇;9～15min,48%～53%甲醇;15～24min,53%～80%甲醇;24～30min,80%～100%甲醇);检测波长为280nm。结果:牛蒡苷及牛蒡苷元的平均回收率分别为97.7%,96.9%。RSD分别为1.3%,0.9%(n=5);线性方程分别为Y=782.6X-64.11(r=0.9998),Y=1220.217X-35.26(r=0.9998)。结论:该方法快速准确,可同时测定牛蒡提取物中牛蒡苷和牛蒡苷元的含量,可用作牛蒡子提取物的质量控制方法。

Objective： To establish a HPLC method for the content determination of arctiin and arctigenin in the extract of Fructus Arctii. Methods： HPLC method was used for the content determination of arctiin and arctigenin. The conditions for HPLC： Column with Kromasil C18 100A（ 150mm ×4. 6mm, 5μm） . Eluting with mixture of methanol-water in gradient： （0-7min, 35% -45% methanol; 7-9min, 45% -48% methanol; 9- 15min, 48% -53% methanol; 15 - 24min, 53% - 80% methanol; 24 - 30min, 80% - 100% methanol） . Detection wavelength was at 280 nm. Results： The average recoveries of arctiin and arctigenin were 97.7% and 96.9% , RSD were 1.3% and 0.9% （n = 5） respectively. The linear equations were Y = 782.6X - 64. 11 （r = 0. 999 8 ） and Y = 1 220. 217X - 35.26 （ r = 0. 999 8 ） . Conclusion ： The HPLC method is rapid and accurate and can be used for the quality control of the extract of Fructus Arctii.