p16蛋白和p27蛋白在恶性骨肿瘤中的表达及其与预后的关系

A Study of the Relation of p16 and p27 Protein to Pathological Character and Prognosis of Chondrosarcoma and Osteosarcoma

目的探讨p16和p27蛋白在恶性骨肿瘤组织中的表达及其与肿瘤的发生、发展及预后的关系。方法应用免疫组织化学S-P法,检测48例软骨肉瘤、68例骨肉瘤,20例软骨瘤做对照;对p16蛋白在软骨肉瘤及p27蛋白在骨肉瘤不同病理分级、临床分期、预后分组中的表达进行对比分析和相关研究。结果软骨肉瘤组织中p16蛋白高表达(++)率与软骨瘤相比,差异有统计学意义(P<0.01)。软骨肉瘤复发组p16蛋白的高表达率与未复发组相比,差异有统计学意义(P<0.05)。p16蛋白高表达率与瘤组织分化程度呈正相关(P<0.01),在软骨肉瘤不同组织学分级间p16蛋白高表达率相比差异均有统计学意义(P<0.05)。骨肉瘤组织中p27蛋白高表达(++)率与软骨瘤相比,差异有统计学意义(P<0.01)。骨肉瘤低生存组p27蛋白高表达率与高生存组相比,差异有统计学意义(P<0.01)。p27表达与瘤组织分化程度呈正相关(P<0.01),与临床分期呈负相关(P<0.01)。结论p16蛋白减少或缺失是软骨肉瘤发生、发展及不良预后的重要指标,p27蛋白减少或缺失是骨肉瘤的发生、发展及不良预后的重要指标,对临床具有重要的指导意义。

Objective To explore the expressions of p16 and p27 protein in chondrosarcoma and osteosarcoma and its relation to the genesis, development and prognosis. Methods Using immunohistochemistry test（ S-P methods） , we examined the expressions of p16 protein in 48 cases with chondrosarcoma and p27 protein in 68 cases with osteosarcoma, 20 cases with osteochondroma as control group. It was compared and relevantly researched to different pathological features, clinical stage and prognosis. Results The strong positive expression rates of p16 and p27 protein in the tissues of chondrosarcoma and osteosarcoma, that in osteochondroma, there were significant difference between the two groups （ P 〈 0.01 ） ; the high expression of p16 protein in the relapsed groups of chondrosarcoma and that in no relapsed groups, there were significant difference between the two groups （ P 〈 0.01 ）. The expression of p16 protein in choudrosarcoma was positively correlated with histological grade （P 〈 0.01 ）. The strong positive expression rate of p27 protein in short living group of osteosarcoma and long living group, there were significant difference between the two groups （ P 〈 0.01 ）. The expression of p27 protein was positively correlated with histological grade （P 〈 0.01 ） , but was negatively with clinical grade （P 〈 0.01 ）. Conclusions The reduction or adsence of p16 and p27 protein may be an important index in the genesis, development and bad prognosis of chondrosarcoma and osteosarcoma, which is important for guiding clinical treatment.