姜黄素抑制β淀粉样蛋白25～35诱导PC12细胞凋亡的实验研究

Inhibitory effect of Curcumin on apoptosis of PC12 cells induced byβamyloid 25-35

目的探讨姜黄索对β淀粉样蛋白(Aβ)25～35诱导的PC12细胞损伤的保护作用。方法采用不同浓度的Aβ25～35处理分化后的PC12细胞,同时应用姜黄素进行干预,采用四甲基偶氮唑盐法检测细胞活力及代谢状态,采用磷脂酰丝氨酸外翻法检测细胞凋亡,采用流式细胞仪检测细胞凋亡率。结果Aβ25～35处理24h后,PC12细胞活力显著下降,且呈剂量依赖性。姜黄素(5～20μmol/L)对Aβ25～35诱导的细胞凋亡具有明显的抑制作用。与仅以20μmol/L Aβ25～35处理相比,5μmol/L姜黄素+20μmol/L Aβ25～35处理后的细胞凋亡百分比明显降低(P<0.01)。结论Aβ25～35能够诱导PC12细胞凋亡,姜黄素对其诱导的PC12细胞凋亡具有保护作用。

Objective To investigate the protective effect of Curcumin on apoptosis of PC12 cells induced by β amyloid（Aβ） 25-35. Methods Differentiated PC12 cells were treated with different concentrations of Aβ25-35 and Curcumin for 24 h. Cell viability was measured by MTT assay and cell apoptosis was detected by Annexin-V staining and flow cytometry. Results The cell viability was significantly decreased 24 h after treatment with Aβ25-35 （ P 〈 0.01 ）; the decrease was in a dose-dependent manner. Curcumin （5-20 μmol/L） showed obvious inhibitory effect on the apoptosis induced by Aβ25-35. Compared with Aβ25-35 （20 μmol/L） group, cell apoptosis in Curcmin （5 μmol/L） plus Aβ25-35 （20 μmol/L） group was significantly lower （ P 〈 0. 01 ）. Conclusion Aβ25-35 can induce neuronal apoptosis and Cucumin can antagonize the cell apoptosis induced by Aβ25-35. （Shanghai Med J, 2007, 30：843-846）