大孔硅胶反相色谱填料的高温溶剂法合成及其在蛋白质分离上的应用

Synthesis of Macroporous Silica Based Reversed Phase Chromatographic Packing in High Temperature Solvents for Separation of Proteins and Peptides

报道利用高温溶剂法在Ｓｉｎｏｐａｋ－Ｓ大孔硅胶上键合十八烷基，得到可用于蛋白质分离的高效反相色谱填料，合成时间缩短为３～５ｈ。用苯、萘、菲及苯胺评价了该反相柱，其理论塔板数达每米３到５万，能有效地用于蛋白质和多肽混合物的分离。

An improved method was presented for the synthesis of C18 reversed-phase chro- matographic column packing material based on Sinopak-S, a macroporous silica gel. The packing material was synthesized in 3 to 5 h under a higher temperature in Suitable solvents. The material showed a high carbon content on the silica gel Surface and excellent chro- matographic performance. Symmetrical peaks were obtained for the separation of benzene, naphthalene, phenanthrene, aniline and pyridine with a mobile phase of 85:15 methanol/ water solution. 3-5×104 theoretical plates/meter for the reversed-phase column was ob- tained. The column also showed an excellent ability for the separation of proteins and pep- tides in a 30 min-Iinear gradient program from 10 to 80% acetonitrile phosphate buffer solu- tion(pH3.2).