异硫氰酸异鲁米诺的电致化学发光性质及其机理

Properties and Mechanism of Electrochemiluminescence of Isothiocyanatoisoluminol

研究了异硫氰酸异鲁米诺的电致化学发光性质及其反应机理。在ＫＯＨ－ＫＣｌ－Ｈ２Ｏ２（ｐＨ１１．８）介质中，施加三角波脉冲电压使异硫氰酸异鲁米诺产生较强的电致化学发光，其浓度在２．５×１０－１１～１．０ × １０－７ｍｏｌ／Ｌ范围与发光强度成线性关系，检测限为６．２×１０－１２ｍｏｌ／Ｌ．重复１０次测定５．０×１０－９ｍｏｌ／Ｌ异硫氰酸异鲁米诺溶液，相对标准偏差为３．０％。方法用于测定Ａｎｔｉ－ＡＦＰ，取得满意结果。

The properties and mechanism of electrochemiluminescence (ELC) of isothio- cyanatoisoluminol(ITCI) have been studied in the medium of KOH-KCl-H2O2 (pH 1l.8) and under the condition of triangular wave potential. There is a linear relationship between the ECL emission intensity and the concentration of ITCI, the linear calibration range is 2.5×l0-11~1×10-7 mol/L and the detection limit is 6.2×10-l2 mol/L. The relative stan- dard deviation is 3.0% for 5.0×10-9 mol/L ITCI. This method has been used to detect An- ti-AFP with satisfactory results.