高效毛细管电泳-电化学法检测烟酰胺腺嘌呤二核苷酸

Determinaiton of Reduced Form of Nicotinamide Adenine Dinucleotide by Capillary Zone Electrophoresis With Amperometric Detection

报道了一种测定烟酰胺腺嘌呤二核苷酸（ＮＡＤＨ）的毛细管电泳电化学检测方法。采用 ３０ ｃｍ × ２５μｍ的石英毛细管分离 ＮＡＤＨ与脲酸，以微型碳糊电极测定经分离后的ＮＡＤＨ的含量。在 ｐＨ ７．５的磷酸盐缓冲溶液中，标准工作曲线的范围为 １．０～１００μ ｍｏｌ／Ｌ；最低检测浓度为 ０．６０ μｍｏｌ／Ｌ（Ｓ／Ｎ＝３．０）。

A method for the determination of reduced form of nicotinamide adenine dinu- cleotide (NADH) using capillary zone electrophoresis with amperometric detection is de- scribed. NADH and uric acid were separated in a 30 cm ?5 μm I. D. quartz capillary with phosphate buffer (pH 7. 5). Electrochemical detection was carried out with a small carbon paste electrode at the working voltage of +0. 85 V(vs. SCE). The detection limit (S/N=3) was 0. 60 μmol/L and the calibration curve was linear in the range of 1. 0-100 μmol/L.