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根癌农杆菌介导丝状真菌遗传转化因素的研究进展 被引量：9

Advance of the Factors Influencing Agrobacterium tumefaciensis Mediated Fungal Transformation
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摘要 根癌农杆菌介导的丝状真菌遗传转化体系,是当前研究真菌基因组学的有力工具,广泛运用于真菌随机插入突变体库的构建和基因打靶。概述了根癌农杆菌介导的丝菌转化的大致过程,转化效率的影响因素。 Agrobacterium tumefaciensis-mediated transformation（AtMT） become a important tool for functional genomics in fungi.In combination with the advantage of AtMT,this method is applied in random insertion mutagenesis and targeted mutagenesis in filamentous fungi.In this article,we summarized the process of the Agrobacterium tumefaciensis-mediated fungal transformation and the factors influencing AtMT efficiency in filamentous fungi.
作者 张震 杜新法 柴荣耀 孙国昌
机构地区 浙江省农业科学院植物保护与微生物研究所
出处 《微生物学杂志》 CAS CSCD 2007年第6期88-91,共4页 Journal of Microbiology
基金 浙江省科技计划项目(2004C12020-5)
关键词 根癌农杆菌 真菌转化 转化效率 影响因素 Agrobacterium tumefaciensis Fungal transformation Transformation efficiency influencing factors
分类号 Q939.5 [生物学—微生物学]
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  • 2Michielse CB, Hooykaas PJJ, Hondel CAMJJ, et al. Agrobacterium-mediated transformation as a tool for functional genomics in fungi[J]. Curr Genet, 2005, 48: 1-17.
  • 3Groot MJ de, Bundock P, Hooykaas PJ, et al. Agrobacterium tumefaciens-mediated transformation of filamentous fungi [J]. Nat Biotechnol, 1998, 16: 839-842.
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微生物学杂志
2007年 第6期

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