噬菌体展示技术研究进展

Recent Development of Phage Display Technique

噬菌体表面展示技术是一种将外源蛋白或抗体可变区与噬菌体表面特定蛋白质融合并展示于其表面,构建蛋白质或抗体库,并从中筛选特异蛋白质或抗体的基因工程技术。介绍这一技术的原理、相关展示系统以及在蛋白质相互作用的研究,抗体及疫苗的制备、多肽药物的研制等方面的应用潜力和独特的优点。

Phage display is a genetic engineering technique that combines the exogenous protein or alternative segment of antibody with the specific surface protein of phage to form peptide or antibody libraries for screening the specific proteins or antibodies.This paper introduced the principle and the relative systems of phages display technique.This technique has unique advantages and great potentials in the many fields such as research of protein interaction and developments of antibodies,vaccine and drugs.