哮喘患者痰液 血清中细胞因子水平与肺功能的相关性研究

Study of the relationship between cell factors and FEV1% in asthmatics patients

目的探讨哮喘患者发作期血清和痰液中炎性反应介质含量与哮喘患者肺功能的关系。方法用高渗盐水雾化引痰，荧光免疫法及酶联免疫法检测发作期哮喘患者痰液和血清中自细胞介素8（IL-8）、肿瘤坏死因子（TNF—α）和嗜酸细胞阳离子蛋白（ECP）水平，同时测定1s用力呼气容积占预计值百分比（FEV1％）。结果哮喘患者血清及痰液中IL-8、TNF—α和ECP等水平均高于健康对照组，并且IL-8、ECP水平均与FEV1％呈负相关。结论测定血清及痰液中的炎性反应介质IL-8、TNF-α和ECP等水平可用于评估哮喘气道炎性反应，血清测定更方便实用，而且通过IL-8和ECP能评估气道阻塞情况。

Objective To assess relationship between lung function and the levels of inflammation factors of the induced sputum and that of serum in asthmatics patients at stage of acute attack. Methods Sputum was induced by inhalation of hypertonic saline. The concentrations of inter-leukin-8（IL-8）, turnout necrosis factor-α（TNF-α） and eosinophil cationic protein （ECP） were assayed by radioimmunoassay （RIA） or enzyme linked immunosorbent （ELISA）, the forced expiratory volume in 1 second expressed as percent predicted（PEV1% ） was also determined at the same time. Results The concentration levels of IL-8,TNF-α and ECP in sputum and serum were higher than the control group. There was negative correlation between IL-8,ECP and FEV1%. Conclusion The concentration levels of ECP, IL-8 and TNF-α in sputum can be used to assess the airway inflammation of asthmatic patients. The airway obstruction can also be assessed by the concentration levels of IL-8 and ECP.