核受体相关因子1基因转染对神经干细胞体外分化的影响

Effect of transfection of NURR1 gene on ex vivo differentiation of neural stem cell

目的研究核受体相关因子1（NURR1）基因对神经干细胞体外分化的影响。方法收集C17．2细胞和pAdeasy-NURR14-C17．2细胞，分别滴加至皿底铺满盖玻片的7cm培养皿中，每组10个培养皿。含血清培养液培养4h细胞贴壁后换无血清培养基继续培养3d，固定。分别行巢蛋白（Nestin）、神经元特异烯醇化酶（NSE）免疫组织化学检测，计数阳性细胞比例。结果C17．2细胞、pAdeasy-NURR1＋C17．2细胞体外无血清培养细胞爬片，Nestin免疫组织化学检测阳性细胞率分别为（15.25±2．45）％、（16.27±1．77）％，差异无统计学意义（P〉0．05）；NSE免疫组织化学检测阳性细胞率分别为（21．48±0．72）％、（70．28±2．14）％，差异有统计学意义（P〈0．05）。结论NURR1基因转染能促进C17．2神经干细胞向神经元方向分化。

Objective To observe the effect of differentiation of neural stem cell transfected by NURR1 gene. Methods Collect C17.2 cells and pAdeasy-NURR1 ＋ C17.2 cells and drip them to the seven-centiliter petri dish, the floor of which was paved with coverglasses （conducted by Poly-L-Lysine）. Each group has ten petri dishes, The cells were cultured in the medium contained blood serum for four hours. When the cells paste the wall, change the medium without blood serum and continue to culture for three days and fix them. Carry out Nestin and NSE immunohistochemical test and count the number of positive cells. Results After Nestin testing of C17.2 cell slides and pAdeasy-NURR1 ＋ C17.2 cell slides cultured in the medium without blood serum, the positive rate was （15.25± 2.45 ） % and （ 16.27 ± 1.77） %, which has no statistic difference（ P 〉 0.05 ） ; while in the NSE test the positive rate was （21.48 ± 0.72） % and （70.28 ± 2.14） % , which has significant difference （ P 〈 0.05 ）. Conclusion Transfection of NURR1 can help C17.2 neural cells differentiate to the neurons type.