部分肠切除大鼠脾气虚证模型量化参数探讨

Study of the quantization parameter using a newly established "Splenic Qi Asthenic Syndrome" model by partial enterectomy in rats

[目的]规范脾气虚证模型的评估标准,建立量化参数。[方法]切除大鼠部分小肠、大肠,建立脾气虚证模型,分为模型组、假手术组,观察造模后第15天腹围指数、粪便含水量、粪便粗蛋白、粗脂肪含量。[结果]模型组腹围指数为0.059±0.01,粪便含水量为(78.30±8.28)%,粪便粗蛋白含量为(31.60±2.75)%,均显著高于假手术组(均P<0.01),粗脂肪含量为(6.50±0.78)%,亦显著高于假手术组(P<0.05)。[结论]腹围指数、粪便中水分、蛋白、脂肪含量能准确反映出腹胀、腹泻、便溏、脾失健运、清阳不升的程度,可作为脾气虚证模型的量化参数。

[Objective]To specify the evaluation standard about animal model of splenic qi asthenic syndrome （SQAS） and establish the Quantization Parameters. [Methods]SQAS model was established in rat by resecting distal ileum, cecum and colon. The rats were divided randomly into model group and sham operation group to observe the index of abdominal girth and water content of feces, measure the stool crude protein and crude fat 15 days later. [Results] The index of abdominal girth in the model group was 0. 059 ±0. 01（cm/g）, significantly higher than that in higher the sham operation group（P〈0.01）. The water content of feces in the model group was （78. 30 ±8. 28）%, significantly higher than that in the sham operation group（P〈0.01）. The crude prorein content of feces was （31. 60 ± 2. 75） %, higher than that in the sham operation group （P〈 0.01）. The crude fat content of feces was（6. 50±0. 78）%,exceeded than that in sham operation group（P〈0. 05）. [Conclusion ]The index of abdominal girth, the water as well as crude protein and crude fat content of feces could accurately reflect the extent of abdominal distention, diarrhea, loose stool,dyssplenism and unrising of lucid yang. So these measurements can be the quantization parameters of ＂Splenic Qi Asthenic Syndrome＂ Mode.