酿酒酵母谷胱甘肽摇瓶发酵研究

Study on Synthesis of Glutathione by Saccharomyces Cerevisiae

通过正交试验,研究了啤酒酵母摇瓶发酵生产谷胱甘肽的培养基的组成以及发酵培养条件。发酵培养基组成研究结果表明,在蔗糖浓度2%、酵母膏浓度1%、(NH4)2SO4浓度0.6%、半胱氨酸浓度为4mmol/L的培养基中,摇床培养36h,GSH产量可达155mg/L。发酵培养条件优化结果表明,在最佳发酵培养基中,发酵时间30h,发酵温度30℃、摇床转速250r/min、接种量3%、250mL三角瓶装液量15mL,谷胱甘肽产量可达248mg/L,比发酵条件优化前提高了37%。

The fermentation culture medium and shaking flask fermentation conditions of glutathione have been researched by using orthogonal experiments with Saccharomyces cerevisiae. The results of media experiment showed as follow： 2% sucrose, 1% yeast extract, 0.6% （NH4）2SO4, 4 mmol/L systeine, shaking flask 36 h, the yield of glutathione reached 155 mg/L in this medium. The results of glutathione fermentation condition experiment showed that the 15 mL broth per 250 mL flask, at 30℃ with reciprocal shaking of 300 r/min under the inoculation of 3% for 30 h. In the optimal fermentation conditions, and glutathione content was up to 248.0 mg/L, which was 37% higher than that of before.